Ym1 protein crystals promote type 2 immunity
| Title: | Ym1 protein crystals promote type 2 immunity |
|---|---|
| Authors: | Ines Heyndrickx, Kim Deswarte, Kenneth Verstraete, Koen HG Verschueren, Ursula Smole, Helena Aegerter, Ann Dansercoer, Hamida Hammad, Savvas N Savvides, Bart N Lambrecht |
| Source: | eLife, Vol 12 (2024) |
| Publisher Information: | eLife Sciences Publications Ltd, 2024. |
| Publication Year: | 2024 |
| Collection: | LCC:Medicine LCC:Science LCC:Biology (General) |
| Subject Terms: | adjuvanticity, protein crystals, Ym1, Ym2, chitinase-like proteins, Charcot-Leyden crystals, Medicine, Science, Biology (General), QH301-705.5 |
| More Details: | Spontaneous protein crystallization is a rare event, yet protein crystals are frequently found in eosinophil-rich inflammation. In humans, Charcot-Leyden crystals (CLCs) are made from galectin-10 (Gal10) protein, an abundant protein in eosinophils. Although mice do not encode Gal10 in their genome, they do form pseudo-CLCs, made from the chitinase-like proteins Ym1 and/or Ym2, encoded by Chil3 and Chil4 and made by myeloid and epithelial cells respectively. Here, we investigated the biological effects of pseudo-CLCs since their function is currently unknown. We produced recombinant Ym1 crystals which were shown to have identical crystal packing and structure by X-ray crystallography as in vivo native crystals derived from murine lung. When administered to the airways of mice, crystalline but not soluble Ym1 stimulated innate and adaptive immunity and acted as a type 2 immune adjuvant for eosinophilic inflammation via triggering of dendritic cells (DCs). Murine Ym1 protein crystals found at sites of eosinophilic inflammation reinforce type 2 immunity and could serve as a surrogate model for studying the biology of human CLCs. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2050-084X |
| Relation: | https://elifesciences.org/articles/90676; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.90676 |
| Access URL: | https://doaj.org/article/027f2b940e04436392626839d4f1ade4 |
| Accession Number: | edsdoj.027f2b940e04436392626839d4f1ade4 |
| Database: | Directory of Open Access Journals |
| ISSN: | 2050084X |
|---|---|
| DOI: | 10.7554/eLife.90676 |
| Published in: | eLife |
| Language: | English |