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Novel electroporation microchip with field constriction enhances transfection efficiency and survival rates of feline embryos

Bibliographic Details
Title: Novel electroporation microchip with field constriction enhances transfection efficiency and survival rates of feline embryos
Authors: Wirakan Kallayanathum, Boonchai Techaumnat, Nitipong Panklang, Witsaroot Sripumkhai, Wutthinan Jeamsaksiri, Okjae Koo, Goo Jang, Theerawat Tharasanit
Source: Scientific Reports, Vol 14, Iss 1, Pp 1-10 (2024)
Publisher Information: Nature Portfolio, 2024.
Publication Year: 2024
Collection: LCC:Medicine
LCC:Science
Subject Terms: Cat, Electroporation, Embryo, Field constriction, Microchip, Resealing time, Medicine, Science
More Details: Abstract This study introduces a low-voltage electroporation microchip designed for transfection in cat embryos, featuring real-time impedance monitoring. The microchip uses a field constriction strategy, which localises the electric field to the membrane region in contact with the micro-orifice, enhancing electroporation efficiency while minimising damage. Embryos were positioned on the orifice, and a series of voltage pulses (10, 15, and 20 V) were applied. Electroporation efficacy was assessed using fluorescent dyes, followed by real-time impedance measurements to monitor the membrane resealing time. It provided valuable insights into membrane recovery times, essential for optimizing gene editing conditions to ensure efficient delivery and maintain cell integrity. The results demonstrated that the microchip with 15 V achieved a 69.5% higher electroporation rate and 100% of survival compared to conventional devices (p
Document Type: article
File Description: electronic resource
Language: English
ISSN: 2045-2322
Relation: https://doaj.org/toc/2045-2322
DOI: 10.1038/s41598-024-80494-z
Access URL: https://doaj.org/article/0274629f6a804caa88046412a048b3ff
Accession Number: edsdoj.0274629f6a804caa88046412a048b3ff
Database: Directory of Open Access Journals
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More Details
ISSN:20452322
DOI:10.1038/s41598-024-80494-z
Published in:Scientific Reports
Language:English