Independent evolution of functionally exchangeable mitochondrial outer membrane import complexes
| Title: | Independent evolution of functionally exchangeable mitochondrial outer membrane import complexes |
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| Authors: | Daniela G Vitali, Sandro Käser, Antonia Kolb, Kai S Dimmer, Andre Schneider, Doron Rapaport |
| Source: | eLife, Vol 7 (2018) |
| Publisher Information: | eLife Sciences Publications Ltd, 2018. |
| Publication Year: | 2018 |
| Collection: | LCC:Medicine LCC:Science LCC:Biology (General) |
| Subject Terms: | mitochondria, trypanosome, outer membrane, MIM complex, biogenesis, Medicine, Science, Biology (General), QH301-705.5 |
| More Details: | Assembly and/or insertion of a subset of mitochondrial outer membrane (MOM) proteins, including subunits of the main MOM translocase, require the fungi-specific Mim1/Mim2 complex. So far it was unclear which proteins accomplish this task in other eukaryotes. Here, we show by reciprocal complementation that the MOM protein pATOM36 of trypanosomes is a functional analogue of yeast Mim1/Mim2 complex, even though these proteins show neither sequence nor topological similarity. Expression of pATOM36 rescues almost all growth, mitochondrial biogenesis, and morphology defects in yeast cells lacking Mim1 and/or Mim2. Conversely, co-expression of Mim1 and Mim2 restores the assembly and/or insertion defects of MOM proteins in trypanosomes ablated for pATOM36. Mim1/Mim2 and pATOM36 form native-like complexes when heterologously expressed, indicating that additional proteins are not part of these structures. Our findings indicate that Mim1/Mim2 and pATOM36 are the products of convergent evolution and arose only after the ancestors of fungi and trypanosomatids diverged. |
| Document Type: | article |
| File Description: | electronic resource |
| Language: | English |
| ISSN: | 2050-084X |
| Relation: | https://elifesciences.org/articles/34488; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.34488 |
| Access URL: | https://doaj.org/article/019892b1610f4591a5fc499fe11a9dd7 |
| Accession Number: | edsdoj.019892b1610f4591a5fc499fe11a9dd7 |
| Database: | Directory of Open Access Journals |
| ISSN: | 2050084X |
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| DOI: | 10.7554/eLife.34488 |
| Published in: | eLife |
| Language: | English |