| Title: |
Elicitation of High-Frequency Cytotoxic T-Lymphocyte Responses against both Dominant and Subdominant Simian-Human Immunodeficiency Virus Epitopes by DNA Vaccination of Rhesus Monkeys |
| Authors: |
Barouch, Dan H., Craiu, Abie, Santra, Sampa, Egan, Michael A., Schmitz, Jo¨rn E., Kuroda, Marcelo J., Fu, Tong-Ming, Nam, Jae-Hwan, Wyatt, Linda S., Lifton, Michelle A., Krivulka, Georgia R., Nickerson, Christine E., Lord, Carol I., Moss, Bernard, Lewis, Mark G., Hirsch, Vanessa M., Shiver, John W., Letvin, Norman L. |
| Source: |
The Journal of Virology; March 2001, Vol. 75 Issue: 5 p2462-2467, 6p |
| Abstract: |
ABSTRACTIncreasing evidence suggests that the generation of cytotoxic T-lymphocyte (CTL) responses specific for a diversity of viral epitopes will be needed for an effective human immunodeficiency virus type 1 (HIV-1) vaccine. Here, we determine the frequencies of CTL responses specific for the simian immunodeficiency virus Gag p11C and HIV-1 Env p41A epitopes in simian-human immunodeficiency virus (SHIV)-infected and vaccinated rhesus monkeys. The p11C-specific CTL response was high frequency and dominant and the p41A-specific CTL response was low frequency and subdominant in both SHIV-infected monkeys and in monkeys vaccinated with recombinant modified vaccinia virus Ankara vectors expressing these viral antigens. Interestingly, we found that plasmid DNA vaccination led to high-frequency CTL responses specific for both of these epitopes. These data demonstrate that plasmid DNA may be useful in eliciting a broad CTL response against multiple epitopes. |
| Database: |
Supplemental Index |
