| Title: |
Gene editing of CD3 epsilonto redirect regulatory T cells for adoptive T cell transfer |
| Authors: |
Du, Weijie, Noyan, Fatih, McCallion, Oliver, Drosdek, Vanessa, Kath, Jonas, Glaser, Viktor, Fuster-Garcia, Carla, Yang, Mingxing, Stein, Maik, Franke, Clemens, Pu, Yaolin, Weber, Olaf, Polansky, Julia K., Cathomen, Toni, Jaeckel, Elmar, Hester, Joanna, Issa, Fadi, Volk, Hans-Dieter, Schmueck-Henneresse, Michael, Reinke, Petra, Wagner, Dimitrios L. |
| Source: |
Molecular Therapy; March 2025, Vol. 33 Issue: 3 p997-1013, 17p |
| Abstract: |
Adoptive transfer of antigen-specific regulatory T cells (Tregs) is a promising strategy to combat immunopathologies in transplantation and autoimmune diseases. However, their low frequency in peripheral blood poses challenges for both manufacturing and clinical application. Chimeric antigen receptors have been used to redirect the specificity of Tregs, using retroviral vectors. However, retroviral gene transfer is costly, time consuming, and raises safety issues. Here, we explored non-viral CRISPR-Cas12a gene editing to redirect Tregs, using human leukocyte antigen (HLA)-A2-specific constructs for proof-of-concept studies in transplantation models. Knock-in of an antigen-binding domain into the N terminus of CD3 epsilon(CD3ε) gene generates Tregs expressing a chimeric CD3ε-T cell receptor fusion construct (TRuC) protein that integrates into the endogenous TCR/CD3 complex. These CD3ε-TRuC Tregs exhibit potent antigen-dependent activation while maintaining responsiveness to TCR/CD3 stimulation. This enables preferential enrichment of TRuC-redirected Tregs over CD3εknockout Tregs via repetitive CD3/CD28 stimulation in a good manufacturing practice-compatible expansion system. CD3ε-TRuC Tregs retained their phenotypic, epigenetic, and functional identity. In a humanized mouse model, HLA-A2-specific CD3ε-TRuC Tregs demonstrate superior protection of allogeneic HLA-A2+skin grafts from rejection compared with polyclonal Tregs. This approach provides a pathway for developing clinical-grade CD3ε-TRuC-based Treg cell products for transplantation immunotherapy and other immunopathologies. |
| Database: |
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