Bibliographic Details
| Title: |
Protocol to reconstruct an in vitro3D full-thickness skin equivalent model with collagen I in 6- and 12-well inserts |
| Authors: |
Tham, Khek-Chian, Lim, Seong Soo, Bonnard, Carine, Common, John E.A. |
| Source: |
STAR Protocols; March 2025, Vol. 6 Issue: 1 |
| Abstract: |
In vitro3D full-thickness reconstituted human skin has high physiological relevance due to the presence of differentiation features often lacking in 2D cell cultures. Here, we present a protocol to reconstruct a 3D skin model using human fibroblasts, keratinocytes, and rat-tail collagen I. We describe steps for cell expansion, the casting of cellular and acellular layers, seeding keratinocytes, the air lifting of culture, and incubation. We also demonstrate the use of chitosan to prevent tissue contraction in 12-well inserts. |
| Database: |
Supplemental Index |
