Bibliographic Details
| Title: |
Impaired 2′,3′-cyclic phosphate tRNA repair causes thermo-sensitive genic male sterility in rice |
| Authors: |
Yan, Bin, Liu, Chunyan, Sun, Jing, Mao, Yang, Zhou, Can, Li, Ji, Liu, Wei, Li, Shengdong, Yan, Wei, Fu, Chenjian, Qin, Peng, Fu, Xingxue, Zhao, Xinghui, Song, Xianwei, Nie, Jiawei, Gao, Feng, Yang, Yuanzhu, Chen, Yuhang, Cao, Xiaofeng |
| Source: |
Cell Research; November 2024, Vol. 34 Issue: 11 p763-775, 13p |
| Abstract: |
Hybrid rice, widely planted in Asia, is pathogen resistant and has superior yields, making it a major contributor to global food security. The two-line hybrid rice system, which utilizes mutants exhibiting photo-/thermo-sensitive genic male sterility (P/TGMS), is the leading hybrid rice breeding technology. Mutations in THERMO-SENSITIVE GENIC MALE STERILE 5(TMS5) accounts for over 95% of current TGMS lines. We previously found that tms5carries a mutation in ribonuclease ZS1. Despite its importance for breeding robust rice lines, the mechanism underlying tms5-mediated TGMS remains elusive. Here, we demonstrate that TMS5 is a tRNA 2′,3′-cyclic phosphatase. The tms5mutation leads to accumulation of 2′,3′-cyclic phosphate (cP)-ΔCCA-tRNAs (tRNAs without 3′ CCA ended with cP), which is exacerbated by high temperatures, and reduction in the abundance of mature tRNAs, particularly alanine tRNAs (tRNA-Alas). Overexpression of tRNA-Alas in the tms5mutant restores male fertility to 70%. Remarkably, male fertility of tms5mutant is completely restored at high temperatures by knocking out OsVms1which encodes the enzyme for cP-ΔCCA-tRNA generation. Our study reveals the mechanism underlying tms5-mediated TGMS in rice and provides mechanistic insight into the further improvement of TGMS in hybrid crop development. |
| Database: |
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