Generation of glioblastoma in mice engrafted with human cytomegalovirus-infected astrocytes
| Title: | Generation of glioblastoma in mice engrafted with human cytomegalovirus-infected astrocytes |
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| Authors: | Guyon, Joris, Haidar Ahmad, Sandy, El Baba, Ranim, Le Quang, Mégane, Bikfalvi, Andreas, Daubon, Thomas, Herbein, Georges |
| Source: | Cancer Gene Therapy; July 2024, Vol. 31 Issue: 7 p1070-1080, 11p |
| Abstract: | Mounting evidence is identifying human cytomegalovirus (HCMV) as a potential oncogenic virus. HCMV has been detected in glioblastoma multiforme (GB). Herewith, we present the first experimental evidence for the generation of CMV-Elicited Glioblastoma Cells(CEGBCs) possessing glioblastoma-like traits that lead to the formation of glioblastoma in orthotopically xenografted mice. In addition to the already reported oncogenic HCMV-DB strain, we isolated three HCMV clinical strains from GB tissues that transformed HAs toward CEGBCs and generated spheroids from CEGBCs that resulted in the appearance of glioblastoma-like tumors in xenografted mice. These tumors were nestin-positive mostly in the invasive part surrounded by GFAP-positive reactive astrocytes. The glioblastoma immunohistochemistry phenotype was confirmed by EGFR and cMet gene amplification in the tumor parallel to the detection of HCMV IE and UL69 genes and proteins. Our results fit with an HCMV-induced glioblastoma model of oncogenesis in vivo which will open the door to new therapeutic approaches and assess the anti-HCMV treatment as well as immunotherapy in fighting GB which is characterized by poor prognosis. |
| Database: | Supplemental Index |
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| Items | – Name: Title Label: Title Group: Ti Data: Generation of glioblastoma in mice engrafted with human cytomegalovirus-infected astrocytes – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Guyon%2C+Joris%22">Guyon, Joris</searchLink><br /><searchLink fieldCode="AR" term="%22Haidar+Ahmad%2C+Sandy%22">Haidar Ahmad, Sandy</searchLink><br /><searchLink fieldCode="AR" term="%22El+Baba%2C+Ranim%22">El Baba, Ranim</searchLink><br /><searchLink fieldCode="AR" term="%22Le+Quang%2C+Mégane%22">Le Quang, Mégane</searchLink><br /><searchLink fieldCode="AR" term="%22Bikfalvi%2C+Andreas%22">Bikfalvi, Andreas</searchLink><br /><searchLink fieldCode="AR" term="%22Daubon%2C+Thomas%22">Daubon, Thomas</searchLink><br /><searchLink fieldCode="AR" term="%22Herbein%2C+Georges%22">Herbein, Georges</searchLink> – Name: TitleSource Label: Source Group: Src Data: Cancer Gene Therapy; July 2024, Vol. 31 Issue: 7 p1070-1080, 11p – Name: Abstract Label: Abstract Group: Ab Data: Mounting evidence is identifying human cytomegalovirus (HCMV) as a potential oncogenic virus. HCMV has been detected in glioblastoma multiforme (GB). Herewith, we present the first experimental evidence for the generation of CMV-Elicited Glioblastoma Cells(CEGBCs) possessing glioblastoma-like traits that lead to the formation of glioblastoma in orthotopically xenografted mice. In addition to the already reported oncogenic HCMV-DB strain, we isolated three HCMV clinical strains from GB tissues that transformed HAs toward CEGBCs and generated spheroids from CEGBCs that resulted in the appearance of glioblastoma-like tumors in xenografted mice. These tumors were nestin-positive mostly in the invasive part surrounded by GFAP-positive reactive astrocytes. The glioblastoma immunohistochemistry phenotype was confirmed by EGFR and cMet gene amplification in the tumor parallel to the detection of HCMV IE and UL69 genes and proteins. Our results fit with an HCMV-induced glioblastoma model of oncogenesis in vivo which will open the door to new therapeutic approaches and assess the anti-HCMV treatment as well as immunotherapy in fighting GB which is characterized by poor prognosis. |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1038/s41417-024-00767-7 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 1070 Titles: – TitleFull: Generation of glioblastoma in mice engrafted with human cytomegalovirus-infected astrocytes Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Guyon, Joris – PersonEntity: Name: NameFull: Haidar Ahmad, Sandy – PersonEntity: Name: NameFull: El Baba, Ranim – PersonEntity: Name: NameFull: Le Quang, Mégane – PersonEntity: Name: NameFull: Bikfalvi, Andreas – PersonEntity: Name: NameFull: Daubon, Thomas – PersonEntity: Name: NameFull: Herbein, Georges IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 07 Text: July 2024 Type: published Y: 2024 Identifiers: – Type: issn-print Value: 09291903 – Type: issn-print Value: 14765500 Numbering: – Type: volume Value: 31 – Type: issue Value: 7 Titles: – TitleFull: Cancer Gene Therapy Type: main |
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