| Title: |
Ultralong transients enhance sensitivity and resolution in Orbitrap-based single-ion mass spectrometry |
| Authors: |
Deslignière, Evolène, Yin, Victor C., Ebberink, Eduard H. T. M., Rolland, Amber D., Barendregt, Arjan, Wörner, Tobias P., Nagornov, Konstantin O., Kozhinov, Anton N., Fort, Kyle L., Tsybin, Yury O., Makarov, Alexander A., Heck, Albert J. R. |
| Source: |
Nature Methods; 20240101, Issue: Preprints p1-4, 4p |
| Abstract: |
Orbitrap-based charge detection mass spectrometry utilizes single-molecule sensitivity to enable mass analysis of even highly heterogeneous, high-mass macromolecular assemblies. For contemporary Orbitrap instruments, the accessible ion detection (recording) times are maximally ~1–2 s. Here by modifying a data acquisition method on an Orbitrap ultrahigh mass range mass spectrometer, we trapped and monitored individual (single) ions for up to 25 s, resulting in a corresponding and huge improvement in signal-to-noise ratio (×5 compared with 1 s), mass resolution (×25) and accuracy in charge and mass determination of Orbitrap-based charge detection mass spectrometry. |
| Database: |
Supplemental Index |
