Bibliographic Details
| Title: |
Ex vivopleural effusion cultures to study chimeric antigen receptor T cell cytotoxicity in an immunocompetent environment |
| Authors: |
Tano, Zachary E., Kiesgen, Stefan, Chintala, Navin K., Dozier, Jordan, Quach, Hue Tu, Messinger, John, Tan, Kay See, Adusumilli, Prasad S. |
| Source: |
Cell Reports Methods; November 2023, Vol. 3 Issue: 11 |
| Abstract: |
Current in vitroand in vivoassays used to study immunotherapeutic interventions lack human immune components that mimic the tumor microenvironment to investigate drug potency and limitations of efficacy. Herein, we describe an ex vivopleural effusion culture (ePEC) assay, using malignant pleural-effusion-derived soluble and cellular factors that differentially affected the cytotoxicity of chimeric antigen receptor (CAR) T cells. Following identification of CAR T cell-suppressive factors, blocking of individual factors reveals their contribution to compromising T cell efficacy. ePEC is a human component assay that can be utilized for developing next-generation cell and antibody therapies that counteract immunosuppression. |
| Database: |
Supplemental Index |
