Bibliographic Details
| Title: |
Novel mouse models based on intersectional genetics to identify and characterize plasmacytoid dendritic cells |
| Authors: |
Valente, Michael, Collinet, Nils, Vu Manh, Thien-Phong, Popoff, Dimitri, Rahmani, Khalissa, Naciri, Karima, Bessou, Gilles, Rua, Rejane, Gil, Laurine, Mionnet, Cyrille, Milpied, Pierre, Tomasello, Elena, Dalod, Marc |
| Source: |
Nature Immunology; April 2023, Vol. 24 Issue: 4 p714-728, 15p |
| Abstract: |
Plasmacytoid dendritic cells (pDCs) are the main source of type I interferon (IFN-I) during viral infections. Their other functions are debated, due to a lack of tools to identify and target them in vivo without affecting pDC-like cells and transitional DCs (tDCs), which harbor overlapping phenotypes and transcriptomes but a higher efficacy for T cell activation. In the present report, we present a reporter mouse, pDC-Tom, designed through intersectional genetics based on unique Siglechand Pacsin1coexpression in pDCs. The pDC-Tom mice specifically tagged pDCs and, on breeding with Zbtb46GFPmice, enabled transcriptomic profiling of all splenic DC types, unraveling diverging activation of pDC-like cells versus tDCs during a viral infection. The pDC-Tom mice also revealed initially similar but later divergent microanatomical relocation of splenic IFN+versus IFN−pDCs during infection. The mouse models and specific gene modules we report here will be useful to delineate the physiological functions of pDCs versus other DC types. |
| Database: |
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