| Title: |
Inference of Active Viral Replication in Cases with Sustained Positive Reverse Transcription-PCR Results for SARS-CoV-2 |
| Authors: |
Rodríguez-Grande, Cristina, Adán-Jiménez, Javier, Catalán, Pilar, Alcalá, Luis, Estévez, Agustín, Muñoz, Patricia, Pérez-Lago, Laura, García de Viedma, Darío |
| Source: |
Journal of Clinical Microbiology; January 2021, Vol. 59 Issue: 2 |
| Abstract: |
The purpose of this study was to detect coronavirus disease 2019 (COVID-19) cases with persistent positive reverse transcription-PCR (RT-PCR) results for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), for which viable virus can be inferred due to the presence of subgenomic (SG) viral RNA, which is expressed only in replicating viruses. RNA remnants purified from diagnostic nasopharyngeal specimens were used as the templates for RT-PCR-specific detection of SG E gene RNA. |
| Database: |
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