Bibliographic Details
| Title: |
Human eosinophils express and release IL‐13 following CD28‐dependent activation |
| Authors: |
Woerly, Gaetane, Lacy, Paige, Younes, Amena Ben, Roger, Nadine, Loiseau, Sylvie, Moqbel, Redwan, Capron, Monique |
| Source: |
Journal of Leukocyte Biology; October 2002, Vol. 72 Issue: 4 p769-779, 11p |
| Abstract: |
Human eosinophils produce a large number of cytokines, including immunoregulatory cytokines. Given that eosinophils store and release interleukin (IL)‐4, a key cytokine in the pathogenesis of allergic inflammation, and that IL‐4 and IL‐13 share common biological functions, we investigated the possibility that IL‐13 may be synthesized by these cells. Using flow cytometry and immunocytochemistry, we show that eosinophils synthesize and store IL‐13. Granule localization was demonstrated after subcellular fractionation, and IL‐13 immunoreactivity was localized to crystalloid, granule‐enriched fractions. Furthermore, electron microscopic analyses specifically localized IL‐13 to the dense cores of bicompartmental secondary granules. Upon CD28 ligation, IL‐13 was released by eosinophils, whereas a combination of CD28 and immunoglobulin A complexes resulted in decreased IL‐13 secretion. Furthermore, eosinophil‐derived IL‐13 exerts a biological effect, inducing CD23 expression on B cells. By having the capacity to synthesize and release IL‐13, eosinophils may participate in the development and maintenance of the T helper cell type 2 response, a prominent feature of allergic diseases. |
| Database: |
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