Bibliographic Details
| Title: |
Gene manipulation using artificial restriction DNA cutter |
| Authors: |
Yamamoto, Yoji, Zhou, Jing-Min, Tomita, Takafumi, Uehara, Akihiko, Aiba, Yuichiro, Watanabe, Akira, Aburatani, Hiroyuki, Komiyama, Makoto |
| Source: |
Nucleic Acids Symposium Series; September 2005, Vol. 49 Issue: 1 p73-73, 1p |
| Abstract: |
Artificial restriction DNA cutter (ARCUT), which we developed recently, was used for manipulation of plasmid DNA. PCR product was inserted into pBR322 plasmid vector using ARCUT, and <it>E. coli</it> cells were transfected with this recombinant plasmid DNA. Successful growth of cells shows that the recombination proceeds without any unexpected mutations. Furthermore, this artificial system was applied to PCR-free construction of chimera protein. |
| Database: |
Supplemental Index |
