Bibliographic Details
| Title: |
Glycerol decreases the integrity of the perinuclear theca in boar sperm. |
| Authors: |
Arenas Núñez, Marco Antonio, Juárez-Mosqueda, María de Lourdes, Gutiérrez-Pérez, Oscar, Anzaldúa Arce, Santiago René, Izquierdo, Alejandro Córdova, Rodríguez, Roberto Martínez, Trujillo Ortega, María Elena |
| Source: |
Zygote; May2013, Vol. 21 Issue 2, p172-177, 6p |
| Subject Terms: |
SPERMATOZOA, GLYCERIN, PROTEASE inhibitors, BOARS, ELECTRON microscopy, LEUPEPTIN, GERM cells, PHYSIOLOGY |
| Abstract: |
We evaluated the effect of glycerol on the perinuclear theca (PT) of boar sperm. Samples from six ejaculates obtained from three different boars were incubated in the detergent Brij 36-T. Spermatozoa were treated with a glycerol concentration of either 2 or 4%, and incubated for 10 or 30 min; two other samples were treated with protease inhibitors (PI; leupeptin or an inhibitor commercial cocktail), mixed with 4% glycerol, and incubated for 30 min. A third glycerol-free group was used as the control. The samples were processed for electron microscopy evaluation. The PT remained intact in 78% of the control samples while, after addition of glycerol for 30 min, the proportion of spermatozoa with disrupted or absent PT increased (P < 0.05). PT was preserved in PI samples, but PT changes increased (P < 0.05). Differences due to treatment with glycerol (2 or 4%) at 10 or 30 min were not observed. These results show, to our knowledge for the first time, the adverse effect of glycerol on the integrity of the PT. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
