| Title: |
Altered T-Cell Responses by the Periodontal Pathogen Porphyromonas gingivalis. |
| Authors: |
Khalaf, Hazem, Bengtsson, Torbjörn, Das, Gobardhan |
| Source: |
PLoS ONE; Sep2012, Vol. 7 Issue 9, Special section p1-9, 9p |
| Subject Terms: |
PERIODONTIUM, ATHEROSCLEROSIS, T cells, LEUPEPTIN, PERIODONTAL disease, CELL communication |
| Abstract: |
Several studies support an association between the chronic inflammatory diseases periodontitis and atherosclerosis with a crucial role for the periodontal pathogen Porphyromonas gingivalis. However, the interplay between this pathogen and the adaptive immune system, including T-cells, is sparsely investigated. Here we used Jurkat T-cells to determine the effects of P. gingivalis on T-cell-mediated adaptive immune responses. We show that viable P. gingivalis targets IL-2 expression at the protein level. Initial cellular events, including ROS production and [Ca2+]i, were elevated in response to P. gingivalis, but AP-1 and NF-κB activity dropped below basal levels and T-cells were unable to sustain stable IL-2 accumulation. IL-2 was partially restored by Leupeptin, but not by Cathepsin B Inhibitor, indicating an involvement of Rgp proteinases in the suppression of IL-2 accumulation. This was further confirmed by purified Rgp that caused a dose-dependent decrease in IL-2 levels. These results provide new insights of how this periodontal pathogen evades the host adaptive immune system by inhibiting IL-2 accumulation and thus attenuating T-cell proliferation and cellular communication. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
