Bibliographic Details
| Title: |
Genome-wide analysis for discovery of rice microRNAs reveals natural antisense microRNAs (nat-miRNAs). |
| Authors: |
Cheng Lu, Dong-Hoon Jeong, Kulkarni, Karthik, Pillay, Manoj, Nobuta, Kan, German, Rana, Thatcher, Shawn R., Maher, Christopher, Lifang Zhang, Ware, Doreen, Bin Liu, Xiaofeng Cao, Meyers, Blake C., Greent, Pamela J. |
| Source: |
Proceedings of the National Academy of Sciences of the United States of America; 3/25/2008, Vol. 105 Issue 12, p4951-4956, 6p, 4 Diagrams, 1 Chart |
| Subject Terms: |
RICE, GENOMES, RNA, GENETIC transcription, ORIGIN of life, GENES |
| Abstract: |
Small RNAs (21-24 nt) are involved in gene regulation through translation inhibition, mRNA cleavage, or directing chromatin modifications. In rice, currently ≈240 microRNAs (miRNAs) have been annotated. We sequenced more than four million small RNAs from rice and identified another 24 miRNA genes. Among these, we `found a unique class of miRNAs that derive from natural cis-antisense transcript pairs. This configuration generates miRNAs that can perfectly match their targets. We provide evidence that `the miRNAs function by inducing mRNA cleavage in the middle of their complementary site. Their production requires Dicer-like 1 (DCL1) activity, which is essential for canonical miRNA biogenesis., All of the natural antisense miRNAs (nat-miRNAs) identified in this study have large introns in their precursors that appear critical for nat-miRNA evolution and for the formation of functional mIRNA loci. These findings suggest that other natural cis-antisense loci with similar exon-intron arrangements could be another source of miRNA genes. [ABSTRACT FROM AUTHOR] |
|
Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Complementary Index |
