Bibliographic Details
| Title: |
CTX-M-15 extended-spectrum -lactamase from Nigerian Klebsiella pneumoniae. |
| Authors: |
Olusegun O. Soge, Anne Marie Queenan, Kayode K. Ojo, Bolanle A. Adeniyi, Marilyn C. Roberts |
| Source: |
Journal of Antimicrobial Chemotherapy (JAC); Jan2006, Vol. 57 Issue 1, p24-30, 7p |
| Abstract: |
Objectives: In this study, extended-spectrum -lactamases (ESBLs) were characterized from 30 selected multidrug-resistant Klebsiella pneumoniae strains isolated from patients with community-acquired urinary tract infections from Southwest Nigeria.Methods: The -lactamases were phenotypically characterized using isoelectric focusing, genotypically characterized using PCR assays and hybridization of the PCR products. Two of the blaCTX-M genes were completely sequenced. The location of the CTX-M-type genes was determined using transformation, DNADNA hybridization, PCR assays and hybridization of the PCR products from the Escherichia coli transformants.Results: All 30 isolates produced at least one -lactamase. Seventeen of the isolates were resistant to cefotaxime, and had =100-fold reduction in susceptibility with cefotaxime plus clavulanic acid (4 mg/L), indicating the presence of an ESBL. The 17 isolates were shown to have blaCTX-M genes that were associated with large plasmids (=58 kb), which also carried a tetracycline resistance gene, tet(A), and various aminoglycoside resistance genes. Two CTX-M-type genes were sequenced and had amino acid sequences indistinguishable from previously sequenced CTX-M-15 -lactamases. The ISEcp1 element was located upstream of blaCTX-M-15 in the same position as previously described. In addition, 23 of the isolates produced TEM -lactamases, 27 produced SHV -lactamases and four produced AmpC -lactamases.Conclusions: Thirty K. pneumoniae produced multiple -lactamases, with 57% producing CTX-M enzymes. This is the first characterization of CTX-M-15-positive K. pneumoniae in Western Africa. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
