Bibliographic Details
| Title: |
The solute-binding component of a putative Mn(II) ABC transporter (MntA) is a novel Bacillus anthracis virulence determinant. |
| Authors: |
Gat, Orit, Mendelson, Itai, Chitlaru, Theodor, Ariel, Naomi, Altboum, Zeev, Levy, Haim, Weiss, Shay, Grosfeld, Haim, Cohen, Sara, Shafferman, Avigdor |
| Source: |
Molecular Microbiology; Oct2005, Vol. 58 Issue 2, p533-551, 19p, 4 Black and White Photographs, 2 Diagrams, 2 Charts, 10 Graphs |
| Subject Terms: |
ATP-binding cassette transporters, CARRIER proteins, MEMBRANE proteins, BIOLOGICAL membranes, PROTEINS, BACILLUS anthracis, BACILLUS (Bacteria), MOLECULAR biology |
| Abstract: |
Here we describe the characterization of a lipoprotein previously proposed as a potential Bacillus anthracis virulence determinant and vaccine candidate. This protein, designated MntA, is the solute-binding component of a manganese ion ATP-binding cassette transporter. Coupled proteomic-serological screen of a fully virulent wild-type B. anthracis Vollum strain, confirmed that MntA is expressed both in vitro and during infection. Expression of MntA is shown to be independent of the virulence plasmids pXO1 and pXO2. An mntA deletion, generated by allelic replacement, results in complete loss of MntA expression and its phenotypic analysis revealed: (i) impaired growth in rich media, alleviated by manganese supplementation; (ii) increased sensitivity to oxidative stress; and (iii) delayed release from cultured macrophages. The Δ mntA mutant expresses the anthrax-associated classical virulence factors, lethal toxin and capsule, in vitro as well as in vivo, and yet the mutation resulted in severe attenuation; a 104-fold drop in LD50 in a guinea pig model. MntA expressed in trans allowed to restore, almost completely, the virulence of the Δ mntA B. anthracis strain. We propose that MntA is a novel B. anthracis virulence determinant essential for the development of anthrax disease, and that B. anthracisΔ mntA strains have the potential to serve as platform for future live attenuated vaccines. [ABSTRACT FROM AUTHOR] |
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| Database: |
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