Bibliographic Details
| Title: |
Addressing unexpected bacterial RNA safety concerns of E. coli produced influenza NP through CpG loaded mutant. |
| Authors: |
Chen, Cen, Li, Mengling, Guo, Aili, Guo, Pengju, Zhang, Wanpo, Gu, Changqin, Wen, Guoyuan, Zhou, Hongbo, Tao, Pan |
| Source: |
NPJ Vaccines; 2/15/2025, Vol. 10 Issue 1, p1-10, 10p |
| Subject Terms: |
BACTERIAL RNA, HEPATITIS B vaccines, ESCHERICHIA coli, INFLUENZA vaccines, INFLUENZA viruses |
| Abstract: |
Influenza virus nucleoprotein (NP) is a promising target for universal influenza vaccines due to its conservation and high immunogenicity. Here, we uncovered a previously unknown factor that E. coli-produced NP carries bacterial RNA, which is crucial for its high immunogenicity but may pose safety and consistency concerns due to batch variability. To address these concerns, we developed a NP mutant (NPmut) that lacks RNA binding activity but can be loaded with CpG1826, a synthetic oligodeoxynucleotide adjuvant that has been used in the FDA-approved Hepatitis B vaccine. The CpG1826-loaded NPmut induced immune responses comparable to RNA-bound NP while eliminating safety risks. Additionally, the mixture of CpG1826-loaded NPmut and 3M2e protein (three tandem copies of the ectodomain of influenza M2 protein) provided enhanced protection against influenza viruses challenge. Our findings highlight the adjuvant activity of bacterial RNA in E. coli-produced NP and propose a safer strategy for developing universal influenza vaccines. [ABSTRACT FROM AUTHOR] |
|
Copyright of NPJ Vaccines is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Complementary Index |
