Bibliographic Details
| Title: |
IFN-? gene expression is controlled by the architectural transcription factor HMGA1. |
| Authors: |
Kai-Yin Chau, Andrea M. Keane-Myers, Monica Fedele, Yasuhiro Ikeda, Remi J. Creusot, Lisa Menozzi, David J. Cousins, Guidalberto Manfioletti, Lionel Feigenbaum, Alfredo Fusco, Santa Jeremy Ono |
| Source: |
International Immunology; Mar2005, Vol. 17 Issue 3, p297-306, 10p |
| Subject Terms: |
CELLS, GENE expression, TRANSCRIPTION factors, TRANSGENIC mice |
| Abstract: |
We report for the first time that IFNG gene expression requires high mobility group (HMG)A1, the architectural transcription factor mediating enhanceosome formation. This finding is supported by our direct studies of T cells isolated from the HMGA1-transgenic mice displaying an up-regulation of IFN-? production and of HMGA1-deficient mice exhibited a decreased IFN-? induction. In parallel transfection studies in EL4 cells, we observed elevated IFNG gene promoter activity in cells stably over-expressing HMGA1 and a reduction of such activity in cells expressing dominant-negative HMGA1. In vitro binding assays further demonstrated a specific interaction of HMGA1 to defined regions of the IFNG gene proximal promoter. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
