| Title: |
CD146 Molecule Expression in B Cells Acute Lymphoblastic Leukemia (B-ALLs): A Flow-Cytometric Marker for an Accurate Diagnostic Workup. |
| Authors: |
Laganà , Alessandro, Totaro, Matteo, Laura Bisegna, Maria, Elia, Loredana, Intoppa, Stefania, Beldinanzi, Marco, Matarazzo, Mabel, di Trani, Mariangela, Costa, Alessandro, Maglione, Raffaele, Mandelli, Biancamaria, Chiaretti, Sabina, Martelli, Maurizio, Stefania De Propris, Maria |
| Source: |
Mediterranean Journal of Hematology & Infectious Diseases; 2024, Vol. 16 Issue 1, p1-9, 9p |
| Subject Terms: |
PHILADELPHIA chromosome, LYMPHOBLASTIC leukemia, PROTEIN-tyrosine kinase inhibitors, ACUTE leukemia, B cells |
| Abstract: |
Background: B-lineage acute lymphoblastic leukemias (B-ALL) harboring the t(9;22)(q34;q11)/BCR::ABL1 rearrangement represent a category with previously dismal prognosis whose management and outcome dramatically changed thanks to the use of tyrosine kinase inhibitors (TKIs) usage and more recently full chemo-free approaches. The prompt identification of these cases represents an important clinical need. Objectives: We sought to identify an optimized cytofluorimetric diagnostic panel to predict the presence of Philadelphia chromosome (Ph) in B-ALL cases by the introduction of CD146 in our multiparametric flow cytometry (MFC) panels. Methods: We prospectively evaluated a total of 245 cases of newly diagnosed B-ALLs with a CD146 positivity threshold >10% referred to the Division of Hematology of 'Sapienza' University of Rome. We compared the results of CD146 expression percentage and its mean fluorescence intensity (MFI) between Ph+ ALLs, Ph-like ALLs, and molecularly negative ALLs. Results: Seventy-nine of the 245 B-ALL cases (32%) did not present mutations at molecular testing, with 144/245 (59%) resulting in Ph+ ALL and 19/245 (8%) Ph-like ALLs. Comparing the 3 groups, we found that Ph+ B-ALLs were characterized by higher expression percentage of myeloid markers such as CD13, CD33, and CD66c and low expression of CD38; Ph+ B-ALL showed a higher CD146 expression percentage and MFI when compared with both molecular negative BALL and Ph-like ALLs; neither the mean percentage of CD146 expression neither CD146 MFI were statically different between molecular negative B-ALL and Ph-like ALLs. Conclusions: Our data demonstrate the association between CD146 expression and Ph+ ALLs. CD146, along with myeloid markers, may help to identify a distinctive immunophenotypic pattern, useful for rapid identification in the diagnostic routine of this subtype of B-ALLs that benefits from a specific therapeutic approach. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
