| Title: |
Pichia fermentans B4-1 phytase with the potential for enhancing soil phosphorous bioavailability. |
| Authors: |
Nankova, Nadezhda, Valkova, Maria, Pisareva, Emiliya, Tomova, Anna, Petrova, Ventsislava |
| Source: |
Bulgarian Journal of Agricultural Science; 2024 Supplement, Vol. 30, p63-68, 6p |
| Subject Terms: |
SOIL mineralogy, PHOSPHORUS in soils, SOIL fertility, PICHIA, PHYTASES, FRUCTOSE, PHYTIC acid |
| Abstract: |
A yeast strain Pichia fermentans B4-1, newly isolated from traditional fermented Bulgarian boza was chosen as a perspective producer of an extracellular phytase to degrade organic phytate and improve soil mineral conditions. The crude enzyme preparation could be applied at temperatures up to 50 °C and pH 5.0. It preserved 100% of its activity after incubation at 45°C for 90 min. In addition to sodium phytate, the P. fermentans B4-1 phytase can hydrolyze a broad range of substrates, such as ATP, glucose 6-phosphate, fructose 1,6-biphosphate, and glycerol 2-phosphate. Partial inhibition of phytase activity was detected (59-73% residual activity) in the presence of mono- and divalent cations like K+, Na+, Zn2+, Ca2+, Cu2+, and Mn2+ at 1 M concentration. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
