| Title: |
Genome-wide evaluation of gene editing outcomes using CRISPR/Cas9 in seed propagated Camelina sativa and vegetatively propagated Solanum tuberosum. |
| Authors: |
Jayakody, Thilani B., Zarka, Daniel, Cho, Keun Ho, Jensen, Jacob, Sikora, Samantha, Buell, C. Robin, Douches, David S., Nadakuduti, Satya Swathi |
| Source: |
Frontiers in Plant Science; 2024, p1-16, 16p |
| Subject Terms: |
SOMATIC mutation, WHOLE genome sequencing, GENOME editing, FOOD crops, TRANSGENIC plants, POTATOES |
| Abstract: |
CRISPR/Cas9 is the most popular genome editing platform for investigating gene function or improving traits in plants. The specificity of gene editing has yet to be evaluated at a genome-wide scale in seed-propagated Camelina sativa (L.) Crantz (camelina) or clonally propagated Solanum tuberosum L. (potato). In this study, seven potato and nine camelina stable transgenic Cas9-edited plants were evaluated for on and off-target editing outcomes using 55x and 60x coverage whole genome shotgun sequencing data, respectively. For both potato and camelina, a prevalence of mosaic somatic edits from constitutive Cas9 expression was discovered as well as evidence of transgenerational editing in camelina. CRISPR/Cas9 editing provided negligible off-target activity compared to background variation in both species. The results from this study guide deployment and risk assessment of genome editing in commercially relevant traits in food crops. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
