Bibliographic Details
| Title: |
Bacterial products initiation of alpha-synuclein pathology: an in vitro study. |
| Authors: |
Ioghen, Octavian Costin, Gaina, Gisela, Lambrescu, Ioana, Manole, Emilia, Pop, Sevinci, Niculescu, Teodora Maria, Mosoia, Oana, Ceafalan, Laura Cristina, Popescu, Bogdan Ovidiu |
| Source: |
Scientific Reports; 12/5/2024, Vol. 14 Issue 1, p1-14, 14p |
| Subject Terms: |
PARKINSON'S disease, MEDICAL sciences, CELL morphology, GUT microbiome, CYTOTOXINS |
| Abstract: |
Parkinson's Disease (PD) is a prevalent and escalating neurodegenerative disorder with significant societal implications. Despite being considered a proteinopathy, in which the aggregation of α-synuclein is the main pathological change, the intricacies of PD initiation remain elusive. Recent evidence suggests a potential link between gut microbiota and PD initiation, emphasizing the need to explore the effects of microbiota-derived molecules on neuronal cells. In this study, we exposed dopaminergic-differentiated SH-SY5Y cells to microbial molecules such as lipopolysaccharide (LPS), rhamnolipid, curli CsgA and phenol soluble modulin α-1 (PSMα1). We assessed cellular viability, cytotoxicity, growth curves and α-synuclein levels by performing MTS, LDH, real-time impedance readings, qRT-PCR and Western Blot assays respectively. Statistical analysis revealed that rhamnolipid exhibited concentration-dependent effects, reducing viability and inducing cytotoxicity at higher concentrations, increasing α-synuclein mRNA and protein levels with negative effects on cell morphology and adhesion. Furthermore, LPS exposure also increased α-synuclein levels. Curli CsgA and PSMα-1 showed minimal or no changes. Our findings suggest that microbiota-derived molecules, particularly rhamnolipid and LPS, impact dopaminergic neurons by increasing α-synuclein levels. This study highlights the potential involvement of gut microbiota in initiating the upregulation of α-synuclein that may further initiate PD, indicating the complex interplay between microbiota and neuronal cells. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
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