Bibliographic Details
| Title: |
Quantification and interpretation of nitric oxide-dependent cutaneous vasodilation during local heating. |
| Authors: |
Wolf, S. Tony, Dillon, Gabrielle A., Alexander, Lacy M., Kenney, W. Larry, Stanhewicz, Anna E. |
| Source: |
Journal of Applied Physiology; Nov2024, Vol. 137 Issue 5, p1418-1424, 7p |
| Subject Terms: |
NITRIC-oxide synthases, METHYL formate, BLOOD flow, VASODILATION, MICRODIALYSIS |
| Abstract: |
Human cutaneous microdialysis approaches for assessing nitric oxide (NO)-dependent blood flow include local heating (LH) of the skin until a plateau is reached, followed by infusion of a NO synthase inhibitor such as NG-nitro- l -arginine methyl ester (l -NAME); however, varied methods of quantifying and expressing NO-dependent vasodilation can obfuscate data interpretation and reproducibility. We retrospectively assessed NO-dependent vasodilation during LH to 39°C or 42°C, calculated as the 1) absolute contribution of the NO-dependent component (along with baseline and the non-NO-dependent component) to the total cutaneous vascular conductance (CVC) response to LH, normalized to maximal CVC (%CVCmax); 2) difference in %CVCmax (Δ%CVCmax) between the LH plateau and post-NO synthase inhibition (l -NAME plateau; Δ%CVCmax = LH plateau − l -NAME plateau); 3) percentage of the LH plateau attributable to Δ%CVCmax (%plateau = Δ%CVCmax/LH plateau × 100); and 4) %plateau when correcting for baseline. The LH plateaus during 39°C and 42°C were 48 ± 17%CVCmax (9 ± 5% baseline; 2 ± 4% non-NO dependent; 36 ± 15% NO dependent) and 88 ± 10%CVCmax (15 ± 8% baseline; 9 ± 10% non-NO dependent; 64 ± 13% NO dependent), respectively. The absolute contributions of the non-NO-dependent and NO-dependent components of the response (P < 0.0001) and the Δ%CVCmax (66 ± 14 vs. 38 ± 15%) were greater during 42°C compared with 39°C (all P ≤ 0.02); however, there were no differences between the two protocols in %plateau (75 ± 13 vs. 80 ± 10%; P = 0.57) or %plateauBL (88 ± 14 vs. 95 ± 8%; P = 0.31). For both protocols, the values were greater for %plateauBL versus Δ%CVCmax and %plateau (P ≤ 0.0001), and for %plateau versus Δ%CVCmax (P ≤ 0.05). Quantification of NO-dependent skin vasodilation responses to LH is dependent upon the mathematical approach and verbal description, which can meaningfully impact data interpretation and reproducibility. NEW & NOTEWORTHY: Local heating protocols are commonly used in conjunction with intradermal microdialysis for assessing nitric oxide (NO)-dependent microvascular function in humans, but various methods used to quantify and describe NO-dependent vasodilation may impact data interpretation. We compared four approaches for quantifying NO-dependent cutaneous vasodilation during local heating at 39°C and 42°C. We identify discrepancies in calculated NO-dependent dilation responses that are dependent upon the mathematical approach and meaningfully impact data interpretation and reproducibility. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
