Bibliographic Details
| Title: |
Integrase strand transfer inhibitors resistance-associated mutations in HIV-infected pregnant women. |
| Alternate Title: |
Resistencia a los inhibidores de integrasa en embarazadas con VIH. (Spanish) |
| Authors: |
Cecchini, Diego, Sfalcin, Javier, Zapiola, Inés, Gomez, Alan, Fernandez-Giuliano, Silvina, Rodriguez, Claudia, Mammana, Lilia, Seravalle, Analía, Fay, Fabián, Torroija, María Cecilia, Bugarín, Gabriela, Bouzas, María Belén |
| Source: |
Revista Española de Quimioterapia; dic2024, Vol. 37 Issue 6, p479-485, 7p |
| Subject Terms: |
INTEGRASE inhibitors, PREGNANT women, HIV, BIOINFORMATICS |
| Geographic Terms: |
LATIN America |
| Abstract (English): |
Objective. To date, no data exist regarding the prevalence of integrase inhibitor (INSTI) resistance-associated mutations (HIVDRM) in HIV-infected pregnant women (HPW) in Latin America. We describe the prevalence and transmissibility of integrase HIVDRM in a historical cohort of INSTI-naïve HPW from Argentina (n=56) with Next Generation Sequencing (NGS). Material and methods. Bioinformatics analysis was performed by HyDRA software for 20%, 10%, 5%, 2%, and 1% sensitivity thresholds. We calculated the mutational viral load for each INSTI-HIVDRM, considering those with >1000 c/mL as of high risk of transmissibility. Results. The predominant HIV subtype was BF (78.5%). Major HIVDRM were not detected with the population sequencing 20% filter. With a 1% threshold, the prevalence increased to 8.9%; Y143C/S, E92G, E138K, and T66I mutations were found. The median (range) mutational load (expressed in c/mL) was: 355 (50.2-1 1705); with only 1 case >1000 c/mL Accessory mutations (G163R/K, T97A) were detected mostly with a 20% sensitivity threshold with an overall prevalence of 23.2%; the median (IQR) mutational load was: 23929 (400963158) c/mL; all of them above 1000 c/mL. Conclusion. Our results show evidence of the presence of major INSTI-HIVDRM as aleatory mutations and a high frequency of accessory mutations with potential transmissibility in HPW. [ABSTRACT FROM AUTHOR] |
| Abstract (Spanish): |
Objetivo. Hasta la fecha, no existen datos sobre la pre-valencia de mutaciones asociadas a la resistencia (HIVDRM) a los inhibidores de la integrasa (INSTI) en mujeres embarazadas infectadas por VIH (HPW) en América Latina. Describimos la prevalencia y la transmisibilidad de las HIVDRM de la integrasa en una cohorte histórica de HPW naive de INSTI de Argentina (n=56) mediante Next Generation Sequencing (NGS). Material y métodos. Se realizó un análisis bioinformático mediante el software HyDRA para umbrales de sensibilidad del 20%, 10%, 5%, 2% y 1%. Calculamos la carga viral mutacional para cada INSTI-HIVDRM, considerando aquellas con >1000 c/mL como de alto riesgo de transmisibilidad. Resultados. El subtipo de VIH predominante fue BF (78,5%). No se detectaron HIVDRM principales con el filtro de secuenciación poblacional del 20%. Con un umbral del 1%, la prevalencia aumentó al 8,9%; se encontraron las mutaciones Y143C/S, E92G, E138K y T66I. La mediana (rango) de la carga mutacional (expresada en c/mL) fue: 355 (50,2-1 1705); con solo 1 caso >1000 c/mL. Las mutaciones accesorias (G163R/K, T97A) se detectaron principalmente con un umbral de sensibilidad del 20%, con una prevalencia general del 23,2%; la mediana (RIQ) de la carga mutacional fue: 23929 (4009-63158) c/mL; todas ellas por encima de 1000 c/mL. Conclusión. Nuestros resultados muestran evidencia de la presencia de INSTI-HIVDRM principales como mutaciones aleatorias y una alta frecuencia de mutaciones accesorias con potencial transmisibilidad en HPW. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
