Bibliographic Details
| Title: |
Post‐Transcriptional Regulation of Rab7a in Lysosomal Positioning and Drug Resistance in Nutrient‐Limited Cancer Cells. |
| Authors: |
Güleç Taşkıran, Aliye Ezgi, Hüsnügil, Hepşen H., Soltani, Zahra E., Oral, Göksu, Menemenli, Nazlı S., Hampel, Chuanpit, Huebner, Kerstin, Erlenbach‐Wuensch, Katharina, Sheraj, Ilir, Schneider‐Stock, Regine, Akyol, Aytekin, Liv, Nalan, Banerjee, Sreeparna |
| Source: |
Traffic; Sep2024, Vol. 25 Issue 9, p1-18, 18p |
| Subject Terms: |
DRUG resistance in cancer cells, CELL populations, CHORIOALLANTOIS, CELL survival, DRUG resistance |
| Abstract: |
Limited nutrient availability in the tumor microenvironment can cause the rewiring of signaling and metabolic networks to confer cancer cells with survival advantages. We show here that the limitation of glucose, glutamine and serum from the culture medium resulted in the survival of a population of cancer cells with high viability and capacity to form tumors in vivo. These cells also displayed a remarkable increase in the abundance and size of lysosomes. Moreover, lysosomes were located mainly in the perinuclear region in nutrient‐limited cells; this translocation was mediated by a rapid post‐transcriptional increase in the key endolysosomal trafficking protein Rab7a. The acidic lysosomes in nutrient‐limited cells could trap weakly basic drugs such as doxorubicin, mediating resistance of the cells to the drug, which could be partially reversed with the lysosomal inhibitor bafilomycin A1. An in vivo chorioallantoic membrane (CAM) assay indicated a remarkable decrease in microtumor volume when nutrient‐limited cells were treated with 5‐Fluorouracil (5‐FU) and bafilomycin A1 compared to cells treated with either agent alone. Overall, our data indicate the activation of complementary pathways with nutrient limitation that can enable cancer cells to survive, proliferate and acquire drug resistance. [ABSTRACT FROM AUTHOR] |
|
Copyright of Traffic is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Complementary Index |
