Bibliographic Details
| Title: |
Stromal Cells Associated with Soft Tissue Augmentation by a Volume-Stable Collagen Matrix (VCMX) Are Predominated by Anti-inflammatory/Reparative Macrophages. |
| Authors: |
Artzi, Zvi, Vered, Marilena, Maymon-Gil, Tal, Renert, Uri, Netanely, Erez, Thoma, Daniel S. |
| Source: |
International Journal of Periodontics & Restorative Dentistry; Sep/Oct2024, Vol. 44 Issue 5, p601-611, 11p |
| Subject Terms: |
IMMUNOPHENOTYPING, MACROPHAGES, TISSUE engineering, ORAL mucosa, LYMPHOCYTES, MANN Whitney U Test, DESCRIPTIVE statistics, BIOMEDICAL materials, FIBROBLASTS, IMMUNOHISTOCHEMISTRY, ANTIGENS, STROMAL cells, ENDOTHELIAL cells, COLLAGEN, DATA analysis software, CONNECTIVE tissues, PHENOTYPES |
| Abstract: |
This article defines immunophenotypes of stromal inflammatory and endothelial cells and fibroblasts 3 months after augmentation of the peri-implant soft tissue using a porcine cross-linked collagen matrix (VCMX). Peri-implant soft tissue samples were obtained from 12 patients at the lining mucosa (LM)-masticatory mucosa (MM) junction before (1) and 3 months after (2) augmentation. Immu-nohistochemical stains were performed to identify inflammatory cells (T [CD3] and B [CD20] lymphocytes, plasma cells [CD138]), macrophages (CD68-proinflammatory, CD163-anti-inflammatory/ reparative), endothelial cells (CD31, CD34), and fibroblasts (CD90, TE-7). Differences in the mean positively stained cells pre- and postaugmentation were analyzed by Wilcoxon signed-rank test. CD31+ endothelial cells showed increased mean numbers in MM2 compared to MM1 (P = .025) and in LM2 compared to LM1 (P = .047). CD163+ anti-inflammatory macrophages showed higher mean numbers in MM2 than in MM1 (P = .021) and in LM2 than in LM1 (P = .012). All other cell phenotypes showed nonsignificant changes between pre- and postaugmentation. This molecular study provides novel insight on the frequency of stromal cell phenotypes in the wound healing process at 3 months postaugmentation with VCMX, with anti-inflammatory CD163+ macrophages being predominant. This should be further investigated to help find novel therapeutic approaches to modulate and promote the VCMX-related healing process. [ABSTRACT FROM AUTHOR] |
|
Copyright of International Journal of Periodontics & Restorative Dentistry is the property of Quintessence Publishing Company Inc. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Complementary Index |
