Bibliographic Details
| Title: |
Single-cell Profiling of Reprogrammed Human Neural Stem Cells Unveils High Similarity to Neural Progenitors in the Developing Central Nervous System. |
| Authors: |
Spathopoulou, Angeliki, Podlesnic, Martina, De Gaetano, Laura, Kirsch, Elena Marie, Tisch, Marcel, Finotello, Francesca, Aigner, Ludwig, Günther, Katharina, Edenhofer, Frank |
| Source: |
Stem Cell Reviews & Reports; Jul2024, Vol. 20 Issue 5, p1325-1339, 15p |
| Subject Terms: |
NEURAL stem cells, EMBRYONIC stem cells, HUMAN stem cells, CENTRAL nervous system, SOMATIC cells |
| Abstract: |
Background: Similar to induced pluripotent cells (iPSCs), induced neural stem cells (iNSCs) can be directly converted from human somatic cells such as dermal fibroblasts and peripheral blood monocytes. While previous studies have demonstrated the resemblance of iNSCs to neural stem cells derived from primary sources and embryonic stem cells, respectively, a comprehensive analysis of the correlation between iNSCs and their physiological counterparts remained to be investigated. Methods: Nowadays, single-cell sequencing technologies provide unique opportunities for in-depth cellular benchmarking of complex cell populations. Our study involves the comprehensive profiling of converted human iNSCs at a single-cell transcriptomic level, alongside conventional methods, like flow cytometry and immunofluorescence stainings. Results: Our results show that the iNSC conversion yields a homogeneous cell population expressing bona fide neural stem cell markers. Extracting transcriptomic signatures from published single cell transcriptomic atlas data and comparison to the iNSC transcriptome reveals resemblance to embryonic neuroepithelial cells of early neurodevelopmental stages observed in vivo at 5 weeks of development. Conclusion: Our data underscore the physiological relevance of directly converted iNSCs, making them a valuable in vitro system for modeling human central nervous system development and establishing translational applications in cell therapy and compound screening. [ABSTRACT FROM AUTHOR] |
|
Copyright of Stem Cell Reviews & Reports is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Complementary Index |
