Bibliographic Details
| Title: |
Protective Effects of Beta-3 Adrenoceptor Agonism on Mucosal Integrity in Hyperoxia-Induced Ileal Alterations. |
| Authors: |
Nardini, Patrizia, Zizi, Virginia, Molino, Marta, Fazi, Camilla, Calvani, Maura, Carrozzo, Francesco, Giuseppetti, Giorgia, Calosi, Laura, Guasti, Daniele, Biagini, Denise, Di Francesco, Fabio, Filippi, Luca, Pini, Alessandro |
| Source: |
Antioxidants; Jul2024, Vol. 13 Issue 7, p863, 18p |
| Subject Terms: |
REACTIVE oxygen species, MORPHOGENESIS, PREMATURE labor, PROTEIN expression, OXIDATIVE stress |
| Abstract: |
Organogenesis occurs in the uterus under low oxygen levels (4%). Preterm birth exposes immature newborns to a hyperoxic environment, which can induce a massive production of reactive oxygen species and potentially affect organ development, leading to diseases such as necrotizing enterocolitis. The β3-adrenoreceptor (β3-AR) has an oxygen-dependent regulatory mechanism, and its activation exerts an antioxidant effect. To test the hypothesis that β3-AR could protect postnatal ileal development from the negative impact of high oxygen levels, Sprague–Dawley rat pups were raised under normoxia (21%) or hyperoxia (85%) for the first 2 weeks after birth and treated or not with BRL37344, a selective β3-AR agonist, at 1, 3, or 6 mg/kg. Hyperoxia alters ileal mucosal morphology, leading to increased cell lipid oxidation byproducts, reduced presence of β3-AR-positive resident cells, decreased junctional protein expression, disrupted brush border, mucin over-production, and impaired vascularization. Treatment with 3 mg/kg of BRL37344 prevented these alterations, although not completely, while the lower 1 mg/kg dose was ineffective, and the higher 6 mg/kg dose was toxic. Our findings indicate the potential of β3-AR agonism as a new therapeutic approach to counteract the hyperoxia-induced ileal alterations and, more generally, the disorders of prematurity related to supra-physiologic oxygen exposure. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
