Structurally diverse zinc(II) complexes containing tripodal tetradentate phenoxido-amines with promising antiproliferative effects.

Bibliographic Details
Title:	Structurally diverse zinc(II) complexes containing tripodal tetradentate phenoxido-amines with promising antiproliferative effects.
Authors:	Massoud, Salah S., Mautner, Franz A., Louka, Febee R., Salem, Nahed M. H., Fischer, Roland C., Torvisco, Ana, Vančo, Ján, Belza, Jan, Dvořák, Zdeněk, Trávníček, Zdeněk
Source:	Dalton Transactions: An International Journal of Inorganic Chemistry; 8/7/2024, Vol. 53 Issue 29, p12261-12280, 20p
Subject Terms:	CELL cycle, CYTOTOXINS, ZINC, LIGANDS (Biochemistry), MEMBRANE potential, MITOCHONDRIAL membranes, LIGANDS (Chemistry), ZINC compounds
Abstract:	Structurally diverse zinc(II) complexes with tripodal tetradentate phenolic-amines of variable substituents in the phenol and amine moieties were synthesized and thoroughly characterized. The two dinuclear [Zn2(L¹)2](ClO4)2·MeOH (1), [Zn2(L²)2](ClO4)2 (2), and four mononuclear [Zn(L³)(H2O)]·MeOH (3), [Zn(L⁴)] (4), [Zn(L⁵)] (5) and [Zn(L⁶)] (6) complexes revealed distorted octahedral, trigonal–bipyramidal or tetrahedral geometries. The free HL¹ and H2L^3–6 ligands, and complexes 1–6 were evaluated for in vitro cytotoxicity against human cancer cell lines (A2780, A2780R, PC-3 and 22Rv1) and normal healthy MRC-5 cells. Overall results revealed high-to-moderate cytotoxicity (with the best IC50 values for complex 6 ranging from 2.4 to 4.5 μM), which is however, significantly higher than that of the reference drug cisplatin. The moderately active complexes 1–4 showed considerable selectivity on A2780 cells (IC50 ≈ 16.3–19.5 μM) over MRC-5 ones (with IC50 >50 μM for 1, 2 and 4, and with IC50 >25 μM for 3). The complexes 1, 2, and 6 and the ligand H2L⁶ were chosen for subsequent deeper biological evaluations. Their time-resolved cellular uptake and other cellular effects in A2780 cells were studied, such as cell cycle profile, intracellular ROS production, induction of apoptosis and activation of caspases 3/7. Complexes 1 and 2 caused significant G0/G1 cell cycle arrest in A2780 cells and antioxidant effects at normal conditions. They showed only limited effects on cellular processes connected with cytotoxicity, i.e. induction of apoptosis, depletion of mitochondrial membrane potential, and autophagy. These findings can be at least partly attributed to the low ability of the complexes to enter the A2780 cells and the depression of metabolic activity of the target cancer cells. [ABSTRACT FROM AUTHOR]
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Database:	Complementary Index

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