Bibliographic Details
| Title: |
Pharmacological potential of Clinacanthus nutans: integrating network pharmacology with experimental studies against lung cancer. |
| Authors: |
Marseti, Salma Wahid, Hermanto, Feri Eko, Widyananda, Muhammad Hermawan, Rosyadah, Nuraini, Kamila, Fairuz Sarah, Annisa, Yuslinda, Dwijayanti, Dinia Rizqi, Ulfa, Siti Mariyah, Widodo, Nashi |
| Source: |
Journal of Biologically Active Products from Nature; Jun2024, Vol. 14 Issue 3, p343-358, 16p |
| Subject Terms: |
LUNG cancer, FLAVONES, MOLECULAR dynamics, PHARMACOLOGY, CYTOTOXINS, FLAVONOIDS |
| Abstract: |
Limited treatment options and emerging resistance necessitate the exploration of novel lung cancer therapeutics. This study investigates the potential of Dandang Gendis (Clinacanthus nutans) leaf extract, a Southeast Asian plant, using a combined in vitro and in silico approach. The in vitro study assessed the extract's cytotoxicity towards A549 lung cancer and TIG-1 fibroblast cells using the WST-1 assay. The in silico analysis identified active compounds, predicted a target protein, performed functional annotation and explored mechanisms through molecular docking and dynamics simulations. The in vitro analysis revealed a significantly higher flavonoid content (177.63 mg QE/g) compared to phenols (45.66 mg GAE/g), suggesting a potential role for flavonoids. Importantly, the extract displayed remarkable selectivity in its cytotoxic effects. It significantly reduced A549 lung cancer cell viability while maintaining high viability in normal TIG-1 fibroblasts, particularly at the 24-hour exposure. While concentrations above 200 μg/ mL significantly decreased the viability of A549 cancer cells, normal TIG-1 fibroblast cells maintained high viability (>50%) at these concentrations. In silico analysis identified isomollupentin 7-O-β-glucoside, isoorientin, and isovitexin, all C-glycosyl flavones, as potential active compounds. These compounds were predicted to act as PD-L1 inhibitors, potentially enhancing T cell-mediated antitumor activity. This aligns with the observed selective cytotoxicity. Overall, this study provides evidence for the potential of the C. nutans extract and its C-glycosyl flavone constituents, particularly the identified compounds, as novel lung cancer therapeutics targeting the PD-L1 pathway for T cell-mediated tumor suppression. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
