Bibliographic Details
| Title: |
ACUTE IMMUNE RESPONSE ANALYSIS OF PACIFIC WHITE SHRIMP (Litopenaeus vannamei) INFECTED BY Vibrio parahaemolyticus AND ITS METABOLITES. |
| Authors: |
Rahayu, Indar Widiastuti, Halalludin, Beni, Herman, Roffi Grandiosa, Dewanti, Lantun Paradhita, Iskandar, Rizkiantino, Rifky, Wisoyo, Daniel, Sari, Putri Purnama, Rahayu, Mufti, Fitriana, Rachmawati Nur, Panjaitan, Bestran Virlando, Haliman, Rubiyanto Widodo, Laiman, Hendri |
| Source: |
Indonesian Journal of Veterinary Sciences / Jurnal Kedokteran Hewan; Mar2024, Vol. 18 Issue 1, p1-7, 7p |
| Subject Terms: |
WHITELEG shrimp, IMMUNE response, BACTERIAL metabolites, PROPHENOLOXIDASE, BACTERIAL cultures |
| Abstract (English): |
This study was aimed to know the Survival Rate (SR) and the acute immune response of Pacific white shrimp (Litopenaeus vannamei), including Total Hemocyte Count (THC), bacterial clearance, and prophenoloxidase (ProPO) activity due to infection with Vibrio parahaemolyticus bacteria and its metabolites. Shrimps were infected by bacterial isolates with culture media (A), bacterial isolates without culture media (B), and metabolites suspensions from bacterial culture media (C) for 8 hours with observation every 2 hours. The results showed that SR of experimental shrimps in group A and B were 41.11±26.43% and 62.22±29.98%, respectively. The shrimp in group C still showed the clinical signs but did not cause the mortality (SR 100.00±0.00%). The THC did not show significant changes, either based on experimental groups or duration of infection, but all groups tended to show decreasing THC at 8 hours post-infection. Bacterial clearance and ProPO activity also did not show significant differences between experimental groups or duration of infection at 8 hours post-infection. However, there was an decrease in bacterial clearance activity from 4 to 6 hours post-infection in the experimental groups A and B. Based on the study results, the acute immune response in shrimps did not show significant changes at 8 hours postinfection caused by any infective material. However, shrimps were infected by bacterial isolates with culture media significantly decreased SR compared with the other two groups. [ABSTRACT FROM AUTHOR] |
| Abstract (Indonesian): |
Penelitian ini bertujuan mengetahui survival rate (SR) dan respon imun akut dari udang putih Pasifik (Litopenaeus vannamei), meliputi total hemocyte count (THC), pembersihan bakteri, dan aktivitas prophenoloxidase (ProPO) akibat infeksi bakteri Vibrio parahaemolyticus dan metabolitnya. Udang diinfeksi isolat bakteri dengan media kultur (A), isolat bakteri tanpa media kultur (B), dan suspensi metabolit dari media kultur bakteri (C) selama 8 jam dengan observasi setiap 2 jam sekali. Hasil penelitian menunjukkan SR udang pada kelompok A dan B masing-masing sebesar 41,11±26,43% dan 62,22±29,98%. Udang pada kelompok C masih menunjukkan gejala klinis namun tidak menyebabkan kematian (SR 100.00±0.00%). Nilai THC tidak menunjukkan perubahan yang signifikan, baik berdasarkan kelompok eksperimen maupun durasi infeksi, namun semua kelompok cenderung menunjukkan penurunan THC pada 8 jam setelah infeksi. Pembersihan bakteri dan aktivitas ProPO juga tidak menunjukkan perbedaan yang signifikan antar kelompok eksperimen atau durasi infeksi pada 8 jam setelah infeksi. Namun terjadi penurunan aktivitas pembersihan bakteri dari 4 menuju 6 jam setelah infeksi pada kelompok A dan B. Berdasarkan hasil penelitian, respons imun akut pada udang tidak menunjukkan perubahan yang signifikan pada 8 jam setelah infeksi yang disebabkan oleh bahan infektif apapun. Namun, udang yang diinfeksi isolat bakteri dengan media kulturnya mengalami penurunan SR secara signifikan dibandingkan dengan dua kelompok lainnya. [ABSTRACT FROM AUTHOR] |
|
Copyright of Indonesian Journal of Veterinary Sciences / Jurnal Kedokteran Hewan is the property of Universitas Syiah Kuala, Faculty of Veterinary Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Complementary Index |
