| Title: |
Sexually dimorphic dynamics of the microtubule network in medaka (Oryzias latipes) germ cells. |
| Authors: |
Mariko Kikuchi, Miyo Yoshimoto, Tokiro Ishikawa, Yuto Kanda, Kazutoshi Mori, Toshiya Nishimura, Minoru Tanaka |
| Source: |
Development (09501991); Mar2024, Vol. 151 Issue 5, p1-9, 9p |
| Subject Terms: |
ORYZIAS latipes, MICROTUBULES, CELL anatomy, GERM cells, SERTOLI cells, GAMETOGENESIS, OOGENESIS, EGGS, SPERMATOZOA |
| Abstract: |
Gametogenesis is the process through which germ cells differentiate into sexually dimorphic gametes, eggs and sperm. In the teleost fish medaka (Oryzias latipes), a germ cell-intrinsic sex determinant, foxl3, triggers germline feminization by activating two genetic pathways that regulate folliculogenesis and meiosis. Here, we identified a pathway involving a dome-shaped microtubule structure that may be the basis of oocyte polarity. This structure was first established in primordial germ cells in both sexes, but was maintained only during oogenesis and was destabilized in differentiating spermatogonia under the influence of Sertoli cells expressing dmrt1. Although foxl3 was dispensable for this pathway, dazl was involved in the persistence of the microtubule dome at the time of gonocyte development. In addition, disruption of the microtubule dome caused dispersal of bucky ball RNA, suggesting the structure may be prerequisite for the Balbiani body. Collectively, the present findings provide mechanistic insight into the establishment of sex-specific polarity through the formation of a microtubule structure in germ cells, as well as clarifying the genetic pathways implementing oocyte-specific characteristics. [ABSTRACT FROM AUTHOR] |
|
Copyright of Development (09501991) is the property of Company of Biologists Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Complementary Index |
