Bibliographic Details
| Title: |
Induction of CD8+ immune memory and enhanced inflammation in a skin inflammation model through pre‐immunization with inactivated pathogens. |
| Authors: |
Falkenberg, Caroline, Bartholdy, Christina, Koch, Janne, Toft, Martin Fitzner, Skov, Søren, Hansen, Camilla Hartmann Friis, Hansen, Axel Kornerup |
| Source: |
CTS: Clinical & Translational Science; Jan2024, Vol. 17 Issue 1, p1-14, 14p |
| Subject Terms: |
SKIN inflammation, IMMUNOLOGIC memory, T cells, PET shops, LABORATORY mice, MICE |
| Abstract: |
Laboratory mice live in specific pathogen‐free (SPF) conditions, resulting in an immature immune system comparable to that of newborns rather than adult humans or mice from pet shops. This condition may compromise their translational value. Reintroducing pathogens would lead to the uncontrolled spread of infections and associated diseases, so research facilities should seek safer alternatives. We immunized laboratory mice with a cocktail of pathogens, which were inactivated by ultraviolet irradiation and mixed with the adjuvant AddaVax. This immunization resulted in a higher percentage of CD8+ effector memory T cells compared to untreated mice, although the response was not as robust as in pet shop mice. In a model of skin inflammation, pre‐immunization led to an increased skin inflammatory response compared to non‐immunized mice. All immunized mice seroconverted to the pathogens in the mixture, while none of the non‐immunized mice housed together seroconverted to the pathogens applied to the pre‐immunized mice. In conclusion, pre‐immunization of mice impacts the immune system, which includes increasing the levels of CD8+ effector memory T cells. [ABSTRACT FROM AUTHOR] |
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Copyright of CTS: Clinical & Translational Science is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Complementary Index |
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