| Title: |
Liquid Biopsy in Alzheimer's Disease Patients Reveals Epigenetic Changes in the PRLHR Gene. |
| Authors: |
Macías, Mónica, Acha, Blanca, Corroza, Jon, Urdánoz-Casado, Amaya, Roldan, Miren, Robles, Maitane, Sánchez-Ruiz de Gordoa, Javier, Erro, María Elena, Jericó, Ivonne, Blanco-Luquin, Idoia, Mendioroz, Maite |
| Source: |
Cells (2073-4409); Dec2023, Vol. 12 Issue 23, p2679, 15p |
| Subject Terms: |
ALZHEIMER'S patients, CIRCULATING tumor DNA, EPIGENETICS, ALZHEIMER'S disease, DNA methylation |
| Abstract: |
In recent years, new DNA methylation variants have been reported in genes biologically relevant to Alzheimer's disease (AD) in human brain tissue. However, this AD-specific epigenetic information remains brain-locked and unreachable during patients' lifetimes. In a previous methylome performed in the hippocampus of 26 AD patients and 12 controls, we found higher methylation levels in AD patients in the promoter region of PRLHR, a gene involved in energy balance regulation. Our aim was to further characterize PRLHR's role in AD and to evaluate if the liquid biopsy technique would provide life access to this brain information in a non-invasive way. First, we extended the methylation mapping of PRLHR and validated previous methylome results via bisulfite cloning sequencing. Next, we observed a positive correlation between PRLHR methylation levels and AD-related neuropathological changes and a decreased expression of PRLHR in AD hippocampus. Then, we managed to replicate the hippocampal methylation differences in plasma cfDNA from an additional cohort of 35 AD patients and 35 controls. The isolation of cfDNA from the plasma of AD patients may constitute a source of potential epigenetic biomarkers to aid AD clinical management. [ABSTRACT FROM AUTHOR] |
|
Copyright of Cells (2073-4409) is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Complementary Index |
|
Full text is not displayed to guests. |
Login for full access.
|
