Bibliographic Details
| Title: |
Pneumocystis jirovecii with high probability detected in bronchoalveolar lavage fluid of chemotherapy-related interstitial pneumonia in patients with lymphoma using metagenomic next-generation sequencing technology. |
| Authors: |
Jin, Dian, Le, Jing, Yang, Qianqian, Cai, Qianqian, Dai, Hui, Luo, Liufei, Tong, Jiaqi, Shu, Wenxiu |
| Source: |
Infectious Agents & Cancer; 12/6/2023, Vol. 18 Issue 1, p1-11, 11p |
| Subject Terms: |
LYMPHOMA diagnosis, CYTOMEGALOVIRUSES, BRONCHOALVEOLAR lavage, SEQUENCE analysis, PNEUMOCYSTIS pneumonia, CANCER chemotherapy, INTERSTITIAL lung diseases, RETROSPECTIVE studies, GENOMES, MICROBIOLOGICAL techniques, EPSTEIN-Barr virus, CHI-squared test, DESCRIPTIVE statistics |
| Abstract: |
Background: Previous studies achieved low microbial detection rates in lymphoma patients with interstitial pneumonia (IP) after chemotherapy. However, the metagenomic next-generation sequencing (mNGS) is a comprehensive approach that is expected to improve the pathogen identification rate. Thus far, reports on the use of mNGS in lymphoma patients with chemotherapy-related IP remain scarce. In this study, we summarized the microbial detection outcomes of lymphoma patients with chemotherapy-related IP through mNGS testing of bronchoalveolar lavage fluid (BALF). Methods: Fifteen lymphoma patients with chemotherapy-related IP were tested for traditional laboratory microbiology, along with the mNGS of BALF. Then, the results of mNGS and traditional laboratory microbiology were compared. Results: Of the 15 enrolled patients, 11 received rituximab and 8 were administered doxorubicin hydrochloride liposome. The overall microbial yield was 93.3% (14/15) for mNGS versus 13.3% (2/15) for traditional culture methods (P ≤ 0.05). The most frequently detected pathogens were Pneumocystis jirovecii (12/15, 80%), Cytomegalovirus (4/15, 26.7%), and Epstein-Barr virus (3/15, 20%). Mixed infections were detected in 10 cases. Five patients recovered after the treatment with antibiotics alone without glucocorticoids. Conclusion: Our findings obtained through mNGS testing of BALF suggested a high microbial detection rate in lymphoma patients with IP after chemotherapy. Notably, there was an especially high detection rate of Pneumocystis jirovecii. The application of mNGS in patients with chemotherapy-related IP was more sensitive. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
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