Extraction-Free RT-PCR Surveillance Testing and Reporting for SARS-CoV-2.

Bibliographic Details
Title: Extraction-Free RT-PCR Surveillance Testing and Reporting for SARS-CoV-2.
Authors: Carney, Patrick R., Duellman, Tyler, Chan, Jia-Yi, Wells, Lauren, Tessmer, Michael, Frater-Rubsam, Leah, Zeller, Molly, Field, Mark, Speers, James, Tyrrell, Kelly, Thompson, Luke, Bondurant, Michael, Morin, Tami, Dagnon, Tamra, Goff, Brian, Runde, Corissa, Splinter-Bondurant, Sandra, Konsitzke, Charles, Kelly, Patrick, Bradfield, Christopher A.
Source: COVID; Jul2023, Vol. 3 Issue 7, p1031-1041, 11p
Subject Terms: COVID-19 pandemic, PUBLIC health, SARS-CoV-2, REVERSE transcriptase polymerase chain reaction, COVID-19 vaccines
Abstract: The COVID-19 pandemic necessitated sensitive, fast, and inexpensive testing for the virus in 2020 prior to the widespread availability of vaccines. Early testing efforts were limited by bottlenecks on reagents, low-throughput testing options, and the slow return of test results. In this paper, we detail the testing pipeline we established at the University of Wisconsin-Madison for rapid, inexpensive, and sensitive surveillance testing for SARS-CoV-2, and we highlight the strengths of the platform that would allow it to be applied to other disease surveillance projects, SARS-CoV-2 variant testing, or future pandemics. This pipeline can be quickly established for further accreditation and clinical application. [ABSTRACT FROM AUTHOR]
Copyright of COVID is the property of MDPI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Complementary Index
More Details
ISSN:26738112
DOI:10.3390/covid3070075
Published in:COVID
Language:English