Bibliographic Details
| Title: |
Expression of miR-31-5p affects growth, migration and invasiveness of papillary thyroid cancer cells. |
| Authors: |
Maggisano, Valentina, Capriglione, Francesca, Verrienti, Antonella, Celano, Marilena, Sponziello, Marialuisa, Pecce, Valeria, Russo, Diego, Durante, Cosimo, Bulotta, Stefania |
| Source: |
Endocrine (1355008X); Mar2023, Vol. 79 Issue 3, p517-526, 10p |
| Abstract: |
Purpose: In this study, we evaluated the biological role of miRNA-31-5p in papillary thyroid cancer (PTC). Methods: By using the real-time PCR, we measured miRNA-31-5p expression levels in 25 PTC tissues and in two human PTC cell lines (K1 and TPC-1). Then, K1 cells were transiently transfected with mirVana inhibitor or mirVana mimic to miRNA-31-5-p. Cell proliferation was determined by MTT and colony formation assays. The in vitro metastatic ability of thyroid cancer cells was evaluated by adhesion, migration and invasion assays. Epithelial mesenchymal transition (EMT) and Hippo pathway related gene and protein levels were evaluated by using the TaqMan™ Gene Expression Assays and western blot analysis, respectively. Results: We found a significant increase of miR-31-5-p expression in tumor tissue and in K1 cells harboring the BRAF p.V600E mutation. Knockdown of miR-31-5p determined a reduction of cell proliferation, associated with a significant decrease in cell adhesion, migration and invasion properties. A downregulation of EMT markers and YAP/β-catenin axis was also observed. Conclusions: Our findings suggest that miRNA-31-5p acts as oncogenic miRNA in human thyrocytes and its overexpression may be involved in the BRAF-related tumorigenesis in PTCs, providing new understanding into its pathological role in PTC progression and invasiveness. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
