| Title: |
Coordinated local RNA overexpression of complement induced by interferon gamma in myositis. |
| Authors: |
Casal-Dominguez, Maria, Pinal-Fernandez, Iago, Pak, Katherine, Muñoz-Braceras, Sandra, Milisenda, Jose C., Torres-Ruiz, Jiram, Dell′Orso, Stefania, Naz, Faiza, Gutierrez-Cruz, Gustavo, Duque-Jaimez, Yaiza, Matas-Garcia, Ana, Valls-Roca, Laura, Garrabou, Gloria, Trallero-Araguas, Ernesto, Walitt, Brian, Christopher-Stine, Lisa, Lloyd, Thomas E., Paik, Julie J., Albayda, Jemima, Corse, Andrea |
| Source: |
Scientific Reports; 2/4/2023, Vol. 13 Issue 1, p1-10, 10p |
| Subject Terms: |
INTERFERON gamma, GENETIC regulation, MYOSITIS, COMPLEMENT receptors, GENE expression, GENETIC overexpression, INTERFERONS, TYPE I interferons |
| Abstract: |
Complement proteins are deposited in the muscles of patients with myositis. However, the local expression and regulation of complement genes within myositis muscle have not been well characterized. In this study, bulk RNA sequencing (RNAseq) analyses of muscle biopsy specimens revealed that complement genes are locally overexpressed and correlate with markers of myositis disease activity, including the expression of interferon-gamma (IFNγ)-induced genes. Single cell and single nuclei RNAseq analyses showed that most local expression of complement genes occurs in macrophages, fibroblasts, and satellite cells, with each cell type expressing different sets of complement genes. Biopsies from immune-mediated necrotizing myopathy patients, who have the lowest levels of IFNγ-induced genes, also had the lowest complement gene expression levels. Furthermore, data from cultured human cells showed that IFNγ upregulates complement expression in macrophages, fibroblasts, and muscle cells. Taken together, our results suggest that in myositis muscle, IFNγ coordinates the local overexpression of complement genes that occurs in several cell types. [ABSTRACT FROM AUTHOR] |
|
Copyright of Scientific Reports is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Complementary Index |
|
Full text is not displayed to guests. |
Login for full access.
|
