Bibliographic Details
| Title: |
Isolation and Pathogenicity of a Novel Goose Astrovirus from Overfed Adult Landaise Geese in China. |
| Authors: |
Zhu, Yinchu, Wang, Hongyu, Hua, Jionggang, Ye, Weicheng, Chen, Liu, Ni, Zheng, Yun, Tao, Ma, Jiale, Yao, Huochun, Bao, Endong, Zhang, Cun |
| Source: |
Viruses (1999-4915); Dec2022, Vol. 14 Issue 12, p2806, 16p |
| Subject Terms: |
GEESE, TRANSMISSION electron microscopy, VIRAL load, WEIGHT loss, ADULTS |
| Geographic Terms: |
ZHEJIANG Sheng (China), CHINA |
| Abstract: |
Goose astrovirus (GAstV) is an important pathogen causing visceral gout and high mortality in goslings, which has broken out and spread across China. In 2021, a disease characterized by urate deposition on the visceral surface and 30% mortality occurred in commercial adult Landaise geese in Zhejiang Province, China. A systematic study identified an infecting astrovirus, designated ZJCX, that was efficiently isolated from a diseased goose with a chicken hepatocellular carcinoma cell line (LMH). In contrast to other GAstVs originating from goslings, ZJCX caused cytopathogenic effects in LMH cells, and the crystalline arrangement of viral particles was observed through transmission electron microscopy. Indeed, phylogenetic analysis and nucleotide homology comparison revealed that ZJCX isolate belongs to the genotype II cluster of GAstVs and displays 97.8–98.4% identity with other GAstV II strains. However, several specific mutations occurred in the polyprotein and capsid protein regions. Moreover, a pathogenicity assessment of ZJCX with a gosling model was conducted, and typical visceral gout was reproduced and led to 18% mortality. The viral loads of ZJCX in the blood, kidney, and liver were detected with specific primers after inoculation, which demonstrated that the kidney and liver presented viral loads peaking at seven days post-inoculation (dpi). Biochemical parameter examination showed that AST, ALT, γ-GT, UA, and BUN levels were significantly increased by GAstV, whereas body weight was reduced. Overall, this study indicated that the GAstV isolate could infect adult geese, and the results regarding the viral loads and biochemical parameters induced by ZJCX provide insight into GAstV pathogenicity. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
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