Bibliographic Details
| Title: |
Proteomic analysis of the actin cortex in interphase and mitosis. |
| Authors: |
Vadnjal, Neza, Nourreddine, Sami, Lavoie, Geneviève, Serres, Murielle, Roux, Philippe P., Paluch, Ewa K. |
| Source: |
Journal of Cell Science; Aug2022, Vol. 135 Issue 16, p1-11, 11p |
| Subject Terms: |
PROTEOMICS, INTERPHASE, ACTIN, CELL morphology, CELLULAR control mechanisms |
| Abstract: |
Many animal cell shape changes are driven by gradients in the contractile tension of the actomyosin cortex, a thin cytoskeletal network supporting the plasmamembrane. Elucidating cortical tension control is thus essential for understanding cell morphogenesis. Increasing evidence shows that alongside myosin activity, actin network organisation and composition are key to cortex tension regulation. However, owing to a poor understanding of how cortex composition changes when tension changes, which cortical components are important remains unclear. In this article, we compared cortices from cells with low and high cortex tensions. We purified cortex-enriched fractions fromcells in interphase andmitosis, asmitosis is characterised by high cortical tension. Mass spectrometry analysis identified 922 proteins consistently represented in both interphase and mitotic cortices. Focusing on actin-related proteins narrowed down the list to 238 candidate regulators of themitotic cortical tension increase. Among these candidates, we found that there is a role for septins in mitotic cell rounding control. Overall, our study provides a comprehensive dataset of candidate cortex regulators, paving the way for systematic investigations of the regulation of cell surface mechanics. [ABSTRACT FROM AUTHOR] |
|
Copyright of Journal of Cell Science is the property of Company of Biologists Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Complementary Index |
