Bibliographic Details
| Title: |
Carbonyl Posttranslational Modification Associated With Early-Onset Type 1 Diabetes Autoimmunity. |
| Authors: |
Yang, Mei-Ling, Connolly, Sean E., Gee, Renelle J., Lam, TuKiet T., Kanyo, Jean, Peng, Jian, Guyer, Perrin, Syed, Farooq, Tse, Hubert M., Clarke, Steven G., Clarke, Catherine F., James, Eddie A., Speake, Cate, Evans-Molina, Carmella, Arvan, Peter, Herold, Kevan C., Wen, Li, Mamula, Mark J. |
| Source: |
Diabetes; Sep2022, Vol. 71 Issue 9, p1979-1993, 15p |
| Subject Terms: |
PROTEIN metabolism, PROTEINS, ANIMAL experimentation, TYPE 1 diabetes, ISLANDS of Langerhans, INSULIN, PROINSULIN, IMMUNITY, RESEARCH funding, MICE, ANTIGENS |
| Abstract: |
Inflammation and oxidative stress in pancreatic islets amplify the appearance of various posttranslational modifications to self-proteins. In this study, we identified a select group of carbonylated islet proteins arising before the onset of hyperglycemia in NOD mice. Of interest, we identified carbonyl modification of the prolyl-4-hydroxylase β subunit (P4Hb) that is responsible for proinsulin folding and trafficking as an autoantigen in both human and murine type 1 diabetes. We found that carbonylated P4Hb is amplified in stressed islets coincident with decreased glucose-stimulated insulin secretion and altered proinsulin-to-insulin ratios. Autoantibodies against P4Hb were detected in prediabetic NOD mice and in early human type 1 diabetes prior to the onset of anti-insulin autoimmunity. Moreover, we identify autoreactive CD4+ T-cell responses toward carbonyl-P4Hb epitopes in the circulation of patients with type 1 diabetes. Our studies provide mechanistic insight into the pathways of proinsulin metabolism and in creating autoantigenic forms of insulin in type 1 diabetes. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
