Bibliographic Details
| Title: |
A Genomic BSAseq Approach for the Characterization of QTLs Underlying Resistance to Fusarium oxysporum in Eggplant. |
| Authors: |
Tassone, Maria Rosaria, Bagnaresi, Paolo, Desiderio, Francesca, Bassolino, Laura, Barchi, Lorenzo, Florio, Francesco Elia, Sunseri, Francesco, Sirangelo, Tiziana Maria, Rotino, Giuseppe Leonardo, Toppino, Laura |
| Source: |
Cells (2073-4409); Aug2022, Vol. 11 Issue 16, p2548, 21p |
| Subject Terms: |
FUSARIUM oxysporum, EGGPLANT, CHROMOSOMES, SPECIES, GENE mapping, GENOMES |
| Abstract: |
Eggplant (Solanum melongena L.), similar to many other crops, suffers from soil-borne diseases, including Fusarium oxysporum f. sp. melongenae (Fom), causing wilting and heavy yield loss. To date, the genetic factors underlying plant responses to Fom are not well known. We previously developed a Recombinant Inbred Lines (RILs) population using as a female parent the fully resistant line '305E40' and as a male parent the partially resistant line '67/3'. The fully resistant trait to Fom was introgressed from the allied species S. aethiopicum. In this work, the RIL population was assessed for the responses to Fom and by using a genomic mapping approach, two major QTLs on chromosomes CH02 and CH11 were identified, associated with the full and partial resistance trait to Fom, respectively. A targeted BSAseq procedure in which Illumina reads bulks of RILs grouped according to their resistance score was aligned to the appropriate reference genomes highlighted differentially enriched regions between resistant/susceptible progeny in the genomic regions underlying both QTLs. The characterization of such regions allowed us to identify the most reliable candidate genes for the two resistance traits. With the aim of revealing exclusive species-specific contigs and scaffolds inherited from the allied species and thus associated with the full resistance trait, a draft de-novo assembly of available Illumina sequences of the '305E40' parent was developed to better resolve the non-recombining genomic region on its CH02 carrying the introgressed Fom resistance locus from S. aethiopicum. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
