Standardization and validation of assay of selected omega-3 and omega-6 fatty acids from phospholipid fraction of red cell membrane using gas chromatography with flame ionization detector.
| Title: | Standardization and validation of assay of selected omega-3 and omega-6 fatty acids from phospholipid fraction of red cell membrane using gas chromatography with flame ionization detector. |
|---|---|
| Authors: | Gupta, Ruby, Dhatwalia, Savita, Chaudhry, Monica, Kondal, Dimple, Stein, Aryeh D., Prabhakaran, Dorairaj, Tandon, Nikhil, Ramakrishnan, Lakshmy, Khandelwal, Shweta |
| Source: | Journal of Analytical Science & Technology; 8/6/2021, Vol. 12 Issue 1, p1-9, 9p |
| Subject Terms: | FLAME ionization detectors, OMEGA-6 fatty acids, OMEGA-3 fatty acids, GAS chromatography, ERYTHROCYTES, DOCOSAHEXAENOIC acid, THIN layer chromatography, FATTY acids |
| Geographic Terms: | INDIA |
| Abstract: | Background: Docosahexaenoic acid (DHA) is an important structural component of human brain and retina. Evidence exists linking nutritional status of pregnant mothers and cognitive functions of their born infants. The DHANI (Maternal DHA Supplementation and Offspring Neurodevelopment in India) trial was implemented to evaluate the effect of maternal supplementation with DHA during pregnancy and for 6 months following delivery on motor and mental development of infants at 1 and 12 months. We describe here the standardization and validation of an assay for measurement of selected omega-3 and omega-6 fatty acids from the phospholipid fraction of red blood cells to assess their status in mothers at baseline, delivery and 6 months post-delivery and for infants in cord blood and at 1 and 12 months of age. The validated method has been used for the analysis of samples for DHANI. Methods: Lipids were extracted from a pool of red blood cells, separated using thin layer chromatography. The phospholipid fraction was esterified, and fatty acids were separated by gas chromatography using a flame ionization detector. Result: The method accuracy for DHA was between 97 - 98% and between 91 - 95% for arachidonic acid (AA) at three different concentrations. The intra-assay and inter-assay coefficient of variation for the fatty acids ranged from 1.19 to 5.7% and 0.78 to 13.0% respectively. Intraclass correlation (ICC), as a measure of reproducibility, ranged between 0.689 and 0.996. A good linearity was observed for all the fatty acids between concentrations of 0.2–4 μg/ml. Conclusion: The standardized and validated method is suitable for implementation in large epidemiological studies for evaluation of fatty acids and in nutritional trials for assessment of fatty acid content of various lipid classes. [ABSTRACT FROM AUTHOR] |
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| Database: | Complementary Index |
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| Items | – Name: Title Label: Title Group: Ti Data: Standardization and validation of assay of selected omega-3 and omega-6 fatty acids from phospholipid fraction of red cell membrane using gas chromatography with flame ionization detector. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Gupta%2C+Ruby%22">Gupta, Ruby</searchLink><br /><searchLink fieldCode="AR" term="%22Dhatwalia%2C+Savita%22">Dhatwalia, Savita</searchLink><br /><searchLink fieldCode="AR" term="%22Chaudhry%2C+Monica%22">Chaudhry, Monica</searchLink><br /><searchLink fieldCode="AR" term="%22Kondal%2C+Dimple%22">Kondal, Dimple</searchLink><br /><searchLink fieldCode="AR" term="%22Stein%2C+Aryeh+D%2E%22">Stein, Aryeh D.</searchLink><br /><searchLink fieldCode="AR" term="%22Prabhakaran%2C+Dorairaj%22">Prabhakaran, Dorairaj</searchLink><br /><searchLink fieldCode="AR" term="%22Tandon%2C+Nikhil%22">Tandon, Nikhil</searchLink><br /><searchLink fieldCode="AR" term="%22Ramakrishnan%2C+Lakshmy%22">Ramakrishnan, Lakshmy</searchLink><br /><searchLink fieldCode="AR" term="%22Khandelwal%2C+Shweta%22">Khandelwal, Shweta</searchLink> – Name: TitleSource Label: Source Group: Src Data: Journal of Analytical Science & Technology; 8/6/2021, Vol. 12 Issue 1, p1-9, 9p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22FLAME+ionization+detectors%22">FLAME ionization detectors</searchLink><br /><searchLink fieldCode="DE" term="%22OMEGA-6+fatty+acids%22">OMEGA-6 fatty acids</searchLink><br /><searchLink fieldCode="DE" term="%22OMEGA-3+fatty+acids%22">OMEGA-3 fatty acids</searchLink><br /><searchLink fieldCode="DE" term="%22GAS+chromatography%22">GAS chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22ERYTHROCYTES%22">ERYTHROCYTES</searchLink><br /><searchLink fieldCode="DE" term="%22DOCOSAHEXAENOIC+acid%22">DOCOSAHEXAENOIC acid</searchLink><br /><searchLink fieldCode="DE" term="%22THIN+layer+chromatography%22">THIN layer chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22FATTY+acids%22">FATTY acids</searchLink> – Name: SubjectGeographic Label: Geographic Terms Group: Su Data: <searchLink fieldCode="DE" term="%22INDIA%22">INDIA</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Background: Docosahexaenoic acid (DHA) is an important structural component of human brain and retina. Evidence exists linking nutritional status of pregnant mothers and cognitive functions of their born infants. The DHANI (Maternal DHA Supplementation and Offspring Neurodevelopment in India) trial was implemented to evaluate the effect of maternal supplementation with DHA during pregnancy and for 6 months following delivery on motor and mental development of infants at 1 and 12 months. We describe here the standardization and validation of an assay for measurement of selected omega-3 and omega-6 fatty acids from the phospholipid fraction of red blood cells to assess their status in mothers at baseline, delivery and 6 months post-delivery and for infants in cord blood and at 1 and 12 months of age. The validated method has been used for the analysis of samples for DHANI. Methods: Lipids were extracted from a pool of red blood cells, separated using thin layer chromatography. The phospholipid fraction was esterified, and fatty acids were separated by gas chromatography using a flame ionization detector. Result: The method accuracy for DHA was between 97 - 98% and between 91 - 95% for arachidonic acid (AA) at three different concentrations. The intra-assay and inter-assay coefficient of variation for the fatty acids ranged from 1.19 to 5.7% and 0.78 to 13.0% respectively. Intraclass correlation (ICC), as a measure of reproducibility, ranged between 0.689 and 0.996. A good linearity was observed for all the fatty acids between concentrations of 0.2–4 μg/ml. Conclusion: The standardized and validated method is suitable for implementation in large epidemiological studies for evaluation of fatty acids and in nutritional trials for assessment of fatty acid content of various lipid classes. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Journal of Analytical Science & Technology is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1186/s40543-021-00287-1 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 1 Subjects: – SubjectFull: INDIA Type: general – SubjectFull: FLAME ionization detectors Type: general – SubjectFull: OMEGA-6 fatty acids Type: general – SubjectFull: OMEGA-3 fatty acids Type: general – SubjectFull: GAS chromatography Type: general – SubjectFull: ERYTHROCYTES Type: general – SubjectFull: DOCOSAHEXAENOIC acid Type: general – SubjectFull: THIN layer chromatography Type: general – SubjectFull: FATTY acids Type: general Titles: – TitleFull: Standardization and validation of assay of selected omega-3 and omega-6 fatty acids from phospholipid fraction of red cell membrane using gas chromatography with flame ionization detector. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Gupta, Ruby – PersonEntity: Name: NameFull: Dhatwalia, Savita – PersonEntity: Name: NameFull: Chaudhry, Monica – PersonEntity: Name: NameFull: Kondal, Dimple – PersonEntity: Name: NameFull: Stein, Aryeh D. – PersonEntity: Name: NameFull: Prabhakaran, Dorairaj – PersonEntity: Name: NameFull: Tandon, Nikhil – PersonEntity: Name: NameFull: Ramakrishnan, Lakshmy – PersonEntity: Name: NameFull: Khandelwal, Shweta IsPartOfRelationships: – BibEntity: Dates: – D: 06 M: 08 Text: 8/6/2021 Type: published Y: 2021 Identifiers: – Type: issn-print Value: 20933134 Numbering: – Type: volume Value: 12 – Type: issue Value: 1 Titles: – TitleFull: Journal of Analytical Science & Technology Type: main |
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