Bibliographic Details
| Title: |
Responses of Lantana Camara Linn. Callus Cultures to Heavy Metals Added to the Culture Media. |
| Authors: |
Tahtamouni, Reham W., Shibli, Rida A. A., Younes, Laila S., Abu-Mallouh, Saida, Al-Qudah, Tamara S. |
| Source: |
Jordan Journal of Biological Sciences; 2020, Vol. 13 Issue 4, p551-557, 7p |
| Subject Terms: |
HEAVY metals, LANTANA camara, CALLUS, GENETIC transformation, CELL suspensions, TISSUE culture |
| Geographic Terms: |
JORDAN |
| Abstract: |
Heavy metals represent a growing threat for ecosystems worldwide. In Jordan, several studies have searched the amounts of heavy metals in soils at roadsides of high ways and soils of litter dumps. The conducted studies found that these soils contained worrying levels of heavy metals that have exceeded in many cases the average world safe limit. For example, lead (Pb) level was 79 mg/kg in soils closed to some highways while the world safe limit is only (25 mg/kg). In other studies, cadmium (Cd) concentration was (5.9 mg/kg) while the world safe limit for Cd is only (0.03 mg/kg). Also in some litter dumps in Jordan, soil chromium (Cr) average content was (6.9 mg/kg) while the world safe limit was only (0.1 mg/kg). Lantana camara Linn. is a flowering plant that has recently attracted the attention of researchers due to its novel phytoremediation powers against some heavy metals, which encouraged some countries to grow them in roadsides of highways and litter dumps. Tissue culture is an excellent approach for studying responses of Lantana camara to heavy metals without interference of other factors. In addition, tissue culture is the main method for producing elite plants lines from callus and cell suspension cultures with superior characteristics by genetic transformation. In this study, callus cultures were induced from leaf discs in vitro. Then, responses of Lantana camara callus cultures to different concentrations (0.0, 0.12, 0.2, 0.3 mg/L) of Cr, Pb, or Cd were monitored under in vitro environmentally controlled conditions to exclude the interference of any other factor. The obtained results revealed that callus growth and quality were found to decrease in response to adding heavy metals to the media at all concentrations. Meanwhile, all callus cultures recorded full survival rates (100%) by the end of the experiments and resumed growth after being transferred to normal growth conditions. Amounts of the three experimented heavy metals (Cr, Pb, and Cd) were found to reach the maximum in callus cultures (0.096, 0.109, 0.0193 mg/kg), when pregrown on Murashige and Skoog media (MS media) supplemented with (0.3 mg/L), while maximum Biological Absorption Coefficient (BAC) values of (0.41, 0.6, 0.30) were recorded in callus cultures pregrown in media with (0.12 mg/L) of either Cr, Pb, or Cd, respectively. More work is still needed to improve BAC values obtained in Lantana camara callus cultures. Also, the produced callus cultures can be used in future research for preparing cell suspension cultures to be introduced to genetic transformation to produce Lantana camara plant lines with hyper accumulation powers against these contaminants. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
