Bibliographic Details
| Title: |
Neutral DNA–avidin nanoparticles as ultrasensitive reporters in immuno-PCR. |
| Authors: |
Chavan, Dimple, Chen, Hui, Crum, Mary, Vu, Binh, Safari, Mohammad, Smith, Maxwell, Vekilov, Peter, Conrad, Jacinta C., Kourentzi, Katerina, Willson, Richard C. |
| Source: |
Analyst; 7/21/2020, Vol. 145 Issue 14, p4942-4949, 8p |
| Subject Terms: |
ETHYLENE glycol, NANOPARTICLES, CHORIONIC gonadotropins, GENE amplification |
| Abstract: |
We have developed an immuno-PCR based diagnostic platform which couples detection antibodies to self-assembled, ultra-detectable DNA–avidin nanoparticles stabilized with poly(ethylene glycol) to link DNA amplification to target protein concentration. Electrostatic neutralization and cloaking of the PCR-amplifiable DNA labels by avidin and PEG coating reduces non-specific "stickiness" and enhances assay sensitivity. We further optimized the detectability of the nanoparticles by incorporating four repeats of a unique synthetic DNA PCR target into each nanoparticle. Using human chorionic gonadotropin hormone (hCG) as a model analyte, this platform was able to quantitate the target hCG protein in femtomolar concentrations using only standard laboratory equipment. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
