Bibliographic Details
| Title: |
Optimizing the sterilization protocol in the process of in vitro cultivation of the genus Vaccinium. |
| Authors: |
Georgieva, Mariya, Georgiev, Diyan, Hristova, Denitsa, Kondakova, Violeta |
| Source: |
Scientific Works of UFT - Plovdiv; 2019, Vol. 66, p93-99, 7p |
| Subject Terms: |
VACCINIUM, STERILIZATION (Disinfection), IN vitro studies, ANTIOXIDANTS, GENOTYPES |
| Abstract: |
The growing interest in Vaccinium (wild species and cultivars) in recent years is due to their extremely valuable antioxidant potential and biochemical composition, as the wild species showing higher values of these indicators than cultivated cultivars. This paper studies the various steps in the protocol for the sterilization of axillary explants of Vaccinium when they are introduced into in vitro conditions, such as origin of the explant, age, sterilizing agent, duration of exposure. Protocol optimization is of great importance for the production of healthy and genetically identical regenerants that can be successfully adapted in in vivo conditions. The study includes: a young growth/shoot of bilberry and lingonberry of different origins - Rhodope Mountains (Yundola, Gela) and the Central Balkan Mountain (Beklemeto), as well as some cultivars, such as 'Brigitta blue' and 'Elliott'. Various exposures (2 min, 2.5 min, 3 min, 3.5 min and 4 min) of one-year axillary explants were used to optimize the sterilization system with sequential sterilizing agents: 70% alcohol and 0.1 % HgCl2 solution. The introduction into in vitro culture was performed on WPM cultural medium supplemented with 3 mg/l 2-iP and pH 4.2, corrected before autoclaving at 1.2 atm. and 121°C for 20 min.Optimizing the sterilization conditions of explants from various Vaccinium species and controlling the morphogenesis of plants grown in vitro is an extremely important element of the protocol for the effective in vitro propagation of selected genotypes for the protection of genetic diversity as well as for selection purposes. [ABSTRACT FROM AUTHOR] |
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| Database: |
Complementary Index |
