Bibliographic Details
| Title: |
High-purity isolation of rare single cells from blood using a tiered microchip system. |
| Authors: |
Gur, Onur, Chang, Chun-Li, Jain, Rohil, Zhong, Yuan, Savran, Cagri A. |
| Source: |
PLoS ONE; 3/17/2020, Vol. 15 Issue 3, p1-20, 20p |
| Subject Terms: |
BLOOD cells, INTEGRATED circuits, CELL lines, BLOOD sampling, LEUKOCYTES |
| Abstract: |
We present a two-tiered microchip system to capture and retrieve rare cells from blood samples with high purity. The first module of the system is a high throughput microfluidic interface that is used to immunomagnetically isolate targeted rare cells from whole blood, and discard > 99.999% of the unwanted leukocytes. The second module is a microwell array that furthers the purification by magnetically guiding each cell into a separate well concurrently, and allows individual retrieval of each cell. We demonstrate the design of the system as well as its characterization by experiments using model cell lines that represent circulating fetal trophoblasts. Our results show that single cells can be retrieved with efficiencies and purities as high as 100% within 145 mins. [ABSTRACT FROM AUTHOR] |
|
Copyright of PLoS ONE is the property of Public Library of Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) |
| Database: |
Complementary Index |
|
Full text is not displayed to guests. |
Login for full access.
|
